TCZB培养液提高小鼠早期胚胎热耐受性的研究#br#

【目的】研究改良的CZB培养液(TCZB)对小鼠早期胚胎发育的影响,寻找一种可以提高动物胚胎热耐受性的新型培养液。【方法】利用原核表达的方法得到TAT蛋白和小鼠Hsp70的串联表达体-Tat-Hsp70,通过在CZB培养液中添加Tat-Hsp70蛋白,配制成TCZB培养液。比较热应激组及对照组胚胎发育率和囊胚孵出率的不同。【结果】小鼠胚胎经39℃热应激处理后,CZB组、TCZB组及对照组的囊胚发育率及囊胚孵出率无显著差异。41℃热应激处理后各组则有显著差异,对照组囊胚发育率及囊胚孵出率(83.7±6.1和75.6±4.5)显著高于TCZB组（81.3±4.5和76.5±7.6）（P＜0.05）,TCZB组显著高于CZB组（80.2±5.4和73.4±6.2）（P＜0.05）。【结论】TCZB培养液可以提高小鼠早期胚胎在41℃强烈热应激条件下的发育率,提高小鼠胚胎的热耐受性。

Enhancement of Heat Tolerance in Mice Early Embryos by TCZB-Culture Medium#br#

【Objective】 The modified CZB medium - TCZB for the development of mouse preimplantation embryos was investigated to find a new medium which could increase the heat tolerance of the embryos. 【Method】 The method of prokaryotic expression was used to get Tat-Hsp70 and this Tat-Hsp70 protein was added to CZB medium to prepare TCZB medium. The embryonic development rate (EDR) and hatching rate (HR) between heat stressed group and control group were compared. 【Result】There was no significant difference in EDR and HR among groups of CZB, TCZB and control after heat stressed at 39℃; however, after treatment at 41℃, both EDR and HR of the control group (83.7±6.1,75.6±4.5) were significantly higher than tnat of both TCZB group (81.3±4.5,76.5±7.6) (P＜0.05) and CZB group (80.2±5.4,73.4±6.2) (P＜0.05), and both EDR and HR in the TCZB group was significantly higher than that of CZB group. 【Conclusion】 TCZB improves both EDR and HR of mouse preimplantation embryos heat stressed at 41℃.