KAP基因的多态性与辽宁绒山羊经济性状的关系研究

 【目的】研究辽宁绒山羊KAP基因的遗传多态性及其多态位点对绒山羊产绒性状和体重性状的关系，寻找可用于标记辅助选择的分子标记，为下一步分子育种提供理论依据。【方法】采用PCR-SSCP技术研究了绒山羊KAP基因的多态性，分析了4个多态位点不同基因型的最小二乘均值（LSM）、最小二乘效应值（LSE）及其遗传方差；通过标记位点与经济性状的遗传相关预测了选择反应。【结果】产绒量性状上，S1-AB和S1-BB的LSM显著高于S1-AA （P＜0.05）,S2-BB 的LSM显著高于S2-AA和S2-AB（P＜0.05），S3-BB的LSM显著高于S3-AA和S3-AB （P＜0.05），S4-AB的LSM显著高于S4-AA和S4-BB（P＜0.05）；在体重性状上，S2-BB的LSM显著高于S2-AA和S2-AB（P＜0.05）,S3-AA和S3-AB的LSM显著高于S3-BB（P＜0.01）；产绒量性状和体重性状上S2位点的选择反应最大。【结论】产绒量性状上，S1-AB，S1-BB，S3-BB和S4-AB基因型可以作为标记基因型；在体重性状上，S3-AA和S3-AB基因型可以作为标记基因型；在体重和产绒量性状上S2-BB基因型可作为多性状标记辅助选择的标记。

Studies of the Relationship Between Polymorphism of KAP Gene and Economic Traits for Liaoning Cashmere Goat

Abstract: 【OBJECTIVE】 Liaoning cashmere goats’ genetic polymorphism of KAP gene was studied and the relationship between polymorphism loci and economic traits （cashmere trait and weight trait） was analyzed in order to find molecular marker of MAS, applying theory basis for next molecular breeding. 【METHOD】 The polymorphism of KAP gene was studied by PCR-SSCP. The least square mean(LSM),the least effect(LSE) and genetic variance of different genotype at 4 polymorphism loci were analyzed, the selection reaction was forecasted by genetic correlation between marker loci and genetic variance of economic traits. 【RESULTS】 In cashmere yield trait, LSM of AA and AB at S1 was significant difference （P<0.05） with BB genotype, BB at S2 was significant difference （P<0.05）with AA and AB genotype ,AA and AB at S3 was significant difference （P<0.05）with BB genotype, AB at S4 was significant difference （P<0.05）with AA and BB genotype; In weight trait , LSM of BB at S2 was significant difference （P<0.05）with AA and AB genotype, AA and AB at S3 was quite significant difference （P<0.01）with AA and AB genotype, BB at S2 was significant difference （P<0.05）with AB and AA genotype. 【CONCLUSION】S1-AB ,S1-BB,S3-BB and S4-AB was the favorable marker genotype for cashmere yield trait, S3-AA was the favorable marker genotype for weight trait, S2-BB was the marker genotype of cashmere yield trait and weight trait Keywords: Liaoning Cashmere goat, KAP, Economic traits