君迁子叶片培养再生植株的研究

 【目的】建立君迁子叶片离体再生体系，为转基因操作奠定基础。【方法】以君迁子幼叶为外植体，研究适合其再生植株的叶片部位、放置方式、基础培养基、植物生长调节剂以及暗培养时间等条件。【结果】叶片基部上表面朝下接种于1/2MS+ ZT 5.0 mg?L-1+IBA 0.01 mg?L-1的培养基，先期暗培养5周后转移至正常光照下培养效果最好，愈伤组织形成率、不定芽再生率和外植体平均不定芽数分别为100.0%、85.3%和2.8±0.6个。再生芽接种于不含植物生长调节剂的1/2MS培养基上，30 d时生根率达100%，平均根数和平均根长分别达5.4条和3.7 cm。再生植株炼苗后移栽，30 d时成活率达98.5%。【结论】成功地建立了君迁子叶片的离体再生体系。

Plant Regeneration from Leaves of Date Plum(Diospyros lotus L.)

Abstract:【Objective】This research aims at developing a plant regeneration system from leaf explants of Diospyros Lotus L. thereby establish a foundation for transformation。【Method】Used tissue culture technique, the conditions for plant regeneration from leaf explants of Diospyros Lotus L. such as leaf segment, inoculate method, basal medium, plant growth regulator and dark period were investigated.【Result】The low segment of leaf with the adaxial surface downwards inoculated on 1/2MS medium containing 0.01 mg?L-1 indole-3-butyric acid(IBA) and 5.0 mg?L-1 zeatin, dark incubated for the first 5 weeks and then transferred to light was the most optimum for shoot regeneration and the shoot percentage and average shoots number per explant were 85.3% and 2.8±0.6 respectively. Regenerated shoots were cut and transferred to 1/2MS medium without any plant growth regulator for rooting. After cultured for one month, the rooting percentage, average roots number and length per shoot reached up to 100%, 5.4 and 3.7 centimeter respectively. After rooting, the regenerated plants were transplant in pots where 98.5% plants survived after one month.【Conclusion】We developed the plant regeneration system from leaf explants of Diospyros Lotus L successfully.