性成熟雄蜂与外勤工蜂触角差异蛋白质组分析

【目的】通过对性成熟雄蜂与外勤工蜂差异蛋白质组进行定量及定性的分析,以期探明雄蜂与工蜂触角发挥生理功能的分子基础。【方法】将性成熟雄蜂与外勤工蜂的触角蛋白质进行双向凝胶电泳以获得蛋白质的分子量、等电点和表达量信息,利用MALDI-TOF质谱以及MASCOT软件对部分表达量有差异的蛋白质进行鉴定,并对其进行表达量的聚类分析。【结果】性成熟雄蜂和外勤工蜂的触角各表达了484、479个蛋白质,其中共有蛋白质416个。在表达的蛋白质中,有102个蛋白质在雄蜂触角中高表达,有80个蛋白质在工蜂触角中高表达。9个已鉴定的差异蛋白质在功能上主要参与转运、激素代谢和能量代谢。雄蜂触角中高表达的有气味结合蛋白质14、脂肪酸结合蛋白质(2个)、保幼激素酯酶(2个)、酰基辅酶A脱氢酶、bellwether异构体1以及CG31974-PA;在工蜂触角中高表达的仅有触角特异蛋白质2。【结论】性成熟雄蜂与外勤工蜂表达的总蛋白质的数量没有明显的差异,且大部分为共有蛋白质,说明工蜂与雄蜂触角的分子进化过程是较为保守的。在雄蜂触角中高表达的参与转运以及代谢的蛋白质,可能是为了使雄蜂婚飞过程中能够更灵敏的接受蜂王的性信息素,准确发现并找到蜂王进行交尾;而在工蜂触角中高表达的触角特异蛋白质2,可能在快速准确寻找蜜粉源、接受巢内外信息素以识别同伴的过程中起着重要作用。

Proteome Comparison Analysis of Sexually Matured Drone and Adult Worker Honevbee Antennae

【Objective】 The goal of this study is to gain better understanding in molecular basis between sexually matured drone and adult worker through differential proteome analysis of their antennae quality and quantity. 【Method】 Two-dimensional gel electrophoresis (2-DE) were run to identify the protein in drone and worker antennae with their molecular weight and pI and expression volume. Accordingly, several proteins with significant variations in their expression volume were identified by using MALDI-TOF/MS and MASCOT software. In addition, hierarchical cluster analysis of the identified proteins was conducted. 【Result】In total, 484 and 479 protein spots were detected in adult drone and worker antennae, respectively, of which 416 expressed proteins were shared between drone and worker antennae. There were 102 and 80 proteins spots upregualted in drone and worker antennae, respectively. Nine proteins spots significantly altered their expression were identified and classified into transport, energy metabolism, hormone metabolism, and a unknown functional group. An odorant binding protein 14, fatty acid binding protein (couple), juvenile hormone esterase (couple), acyl-CoA dehydrogenase, bellwether isoform , CG31974-PA were expressed highly in the antennae of sexually matured drone. An antennal special protein 2 was expressed highly in the antennae of adult worker. 【Conclusion】 Most of the protein spots were commonly expressed in the antennae of the two castes, indicating both castes of honeybees are conservative during molecular evolution through their antennae. Transport and metabolism related proteins were highly expressed in drone antennae indicating they are crucial in assisting the drones to discriminate sex pheromone and chase the queen to make successful mating exactly and swiftly. Antennal special protein 2 was expressed highly in worker antennae which is likely to help the foragers to search nectar resource quickly and recognize its nest mate through receiving colony pheromone.