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| 栽培萝卜种质亲缘关系的AFLP分析 | |
| 引用本文： | 孔秋生,李锡香,向长萍,邱杨,沈镝.栽培萝卜种质亲缘关系的AFLP分析[J].中国农业科学,2005,38(5):1017-1023. |
| 作者姓名： | 孔秋生 李锡香 向长萍 邱杨 沈镝 |
| 作者单位： | 1. 中国农业科学院蔬菜花卉研究所,北京,100081;华中农业大学园艺林学学院,武汉,430070 2. 中国农业科学院蔬菜花卉研究所,北京,100081 3. 华中农业大学园艺林学学院,武汉,430070 |
| 基金项目： | 农业部农作物资源保护资助项目(NB03-6)，“十五”国家科技攻关项目资助(2004BA525B08) |
| 摘 要： | 利用筛选出的8对引物对56份来源于不同国家和地区的栽培萝卜种质的亲缘关系进行了AFLP分析，共扩增出327条带，其中多态性带128条，多态性位点百分率39.1%，显示出栽培萝卜种质之间存在着较丰富的遗传多样性。系统聚类分析将供试材料分为5类9组，主坐标分析将其分为4类7组，2种分类方法所获结果基本一致。基于分子标记的分类与种质的表型基本吻合。 |
| 关 键 词： | 萝卜 Raphanus sativus L. AFLP 聚类分析 主坐标分析 亲缘关系 |
| 收稿时间： | 2004-6-17 |
Phylogenetic Analysis of Cultivated Radish (Raphanus sativus L.) Germplasm with AFLP |
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| KONG Qiu-sheng,LI Xi-xiang,XIANG Chang-ping,QIU Yang,SHEN Di.Phylogenetic Analysis of Cultivated Radish (Raphanus sativus L.) Germplasm with AFLP[J].Scientia Agricultura Sinica,2005,38(5):1017-1023. | |
| Authors: | KONG Qiu-sheng LI Xi-xiang XIANG Chang-ping QIU Yang SHEN Di |
| Institution: | KONG Qiu-sheng 1,2,LI Xi-xiang 1,XIANG Chang-ping 2,QIU Yang 1 ,SHEN Di 1 |
| Abstract: | Phylogenesis of 56 cultivated radish germplasm from different countries or regions was analyzed with amplified fragment length polymorphism (AFLP). A total of 327 bands were detected with 8 pairs of informative AFLP primers among the accessions tested. Of them, 128 bands (39.1%) were polymorphic, indicating abundant genetic diversity existing among the cultivated radish. The 56 cultivars were clustered into 5 groups and 9 subgroups by cluster analysis, and 4 groups and 7 subgroups by principal coordinate analysis. The two methods above exhibited similar phylogenesis among the tested cultivars. The results from molecular classification were almost coincided with germplasm phenotypes. |
| Keywords: | Radish Raphanus sativus L AFLP Cluster analysis Principal coordinate analysis Phylogenesis |
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