小麦糖原合成酶激酶(TaGSK1)的亚细胞定位及其功能鉴定

【目的】对小麦糖原合成酶激酶（TaGSK1）进行亚细胞水平定位以及功能鉴定。【方法】构建Tagsk1-gfp融合基因表达载体并转化拟南芥，以仅携带gfp基因的拟南芥作为对照，利用共聚焦显微镜观察绿色荧光蛋白在转基因植株根部细胞的分布；利用含不同浓度NaCl的MS培养基对携带有融合基因的拟南芥和Columbia型拟南芥进行耐盐性鉴定，观察主根生长量和侧根生长数目。【结果】发现TaGSK1存在于细胞质内，且该激酶在根尖分生组织和与侧根发生有关的中柱鞘细胞中分布较多；耐盐性鉴定结果表明，转Tagsk1-gfp融合基因的植株的平均主根生长量和侧根生长数目与Columbia型拟南芥植株的差异均达到极显著水平（P<0.01）。【结论】TaGSK1定位于细胞质内，该激酶具有促进细胞分裂及提高转基因拟南芥抗渗透、抗胁迫的能力。

Localization and Function Analysis of Wheat Glycogen Syntheses Kinase (TaGSK 1)

【Objective】The localization and function of wheat glycogen synthase kinase (TaGSK1) in plant cells was studied. 【Method】Tagsk1 gene linked with gfp gene was transformed into Arabidopsis via Agrobactiria. Arabidopsis containing gfp was used as control, the distribution of TaGSK1 in root cells was observed with laser confocal microscope. Different transgenic plants were planted in MS medium containing different concentrations of NaCl for screening salt-tolerant plants. Using the wild type Arabidopsis as control, the length of taproots was measured on the seventh day and the lateral roots number was counted on the ninth day. 【Result】 It was found that the green fluorescence of fused protein TaGSK-GFP distributed in cytoplasm of the transgenic plant cells, and mainly in root meristem and stelar sheath, where new roots would develop. Significant difference (P<0.01) existed between transgenic plants and their control plants about the root number and length. 【Conclusion】It is suggested that TaGSK1 localized in cytoplasm and could improve cell proliferation, development, anti-osmotic and anti-stress ability of transgenic plants.