抗戊唑醇草莓枯萎病菌ZY-W的诱导及其生物学特性

【目的】诱导获得草莓枯萎病菌对戊唑醇的抗性菌株,并比较敏、抗菌株在生物学特性方面的差异。【方法】在含戊唑醇PDA平板培养基上,对草莓枯萎病菌进行逐代处理,以诱导其抗性菌株;利用菌落直径测定法测定抗性菌株对其它一些杀菌剂的交互抗性,及其与敏感菌株之间生物学特性差异。【结果】敏感菌株选育至36代,对戊唑醇的抗性达34.22倍,抗性能够稳定遗传;抗戊唑醇菌株对三唑酮等7种药剂表现出了不同程度的交互抗性;抗性菌株与敏感菌株的适宜生长温度均为25℃,适宜pH值为9;敏、抗菌株在不同碳、氮源中,菌丝生长与产孢趋势有所不同。【结论】草莓枯萎病菌在环境中容易形成戊唑醇抗性群体,抗性风险较高,生产中需引起注意。

Induction and Characteristics of Fusarium oxysporum f. Sp. Fragariae ZY-W Resistant to Tebuconazole

【Objective】 The aim of the study is to induce the resistant isolate of Fusarium oxysporum f. sp. fragariae by in vitro cultivation on media containing tebuconazole and compare the biological characteristics between the sensitive and resistant isolates. 【Method】 The resistant isolate was selected on the media containing tebuconazole. By measuring the colony diameters, the characteristics of the resistant and sensitive isolates and the cross resistance of the resistant isolate to some fungicides were studied. 【Result】 The results showed that the resistance of the induced Fusarium oxysporum f. sp. fragariae isolate to tebuconazole reached 34.22 folds after 36-times selection compared with the sensitive isolate. The resistant level of tebuconazole-resistant isolate was stable. Meanwhile, the positive cross resistance to triadimefon and 6 other fungicides was reported. The optimal temperature was 25 ℃, and the optimum pH value was 9 for both sensitive and resistant isolates. The effects of different carbon and nitrogen sources on the growth and the spore production were different. 【Conclusion】 Fusarium oxysporum f. sp. fragariae is susceptible to produce resistant population in environment, and the resistance risk of Fusarium oxysporum f. sp. fragariae needs to be noticed in production.