首页 | 本学科首页   官方微博 | 高级检索  
     检索      

赞皇大枣枣疯病植原体分子分类
引用本文:杨海旭,王洋,赵彦檩,赵锦,刘孟军.赞皇大枣枣疯病植原体分子分类[J].中国农业科学,2011,44(21):4429-4437.
作者姓名:杨海旭  王洋  赵彦檩  赵锦  刘孟军
作者单位:1.河北农业大学中国枣研究中心,河北保定 071001; 2.河北农业大学生命科学学院,河北保定 071000
基金项目:河北自然科学基金项目(C2009000534,08D020); 河北省杰出青年基金项目(C2010000679); 国家科技支撑计划项目(2008BAD92B03,2007BAD36B07,2006BAD18B02)
摘    要: 【目的】枣疯病是一种重要的植原体病害,本试验旨在明确惟一已知的天然三倍体品种--赞皇大枣枣疯病植原体的分类地位,为赞皇大枣枣疯病植原体分类研究提供参考。【方法】利用植原体16S rDNA通用引物对赞皇大枣枣疯病病株的DNA进行PCR扩增和克隆测序,通过BLAST比对进行序列分析,利用临位相连法构建16S rDNA系统演化树,并应用DNAstar软件进行虚拟RFLP分析。【结果】获得的赞皇大枣枣疯病植原体16S rDNA序列长度为1 850 bp(GenBank登录号GU184180),包括1 529 bp的16S rDNA基因全序列、264 bp的邻近间隔区序列及57 bp的23S rDNA部分基因序列。同源分析表明,赞皇大枣枣疯病病原16S rDNA序列与其它植原体的相似性在87%—98%,其中与榆树黄化 Elm yellows(EY)(GenBank登录号l33763)最高相似性为98%;与大多数二倍体枣品种的植原体序列相似率达99%以上;其虚拟RFLP图谱与4个16SrⅤ亚组的代表序列图谱差异显著,赞皇大枣枣疯病植原体延伸因子tuf基因的序列(GenBank登录号JN001985) 比对结果表明,与其它亚组序列同源性为52.3%—76.7%。【结论】三倍体赞皇大枣枣疯病病原属于榆树黄化组(16Sr V),并与其中的B亚组亲缘关系最近;与二倍体品种的病原分类地位一致,说明枣疯病植原体在系统进化上比较保守。

关 键 词:赞皇大枣  枣疯病植原体  16S  rDNA  分子分类
收稿时间:2010-12-16

Molecular Classification of Jujube Witches' Broom(JWB) Associated Phytoplasma from Ziziphus jujuba Mill.'Zanhuangdazao'
YANG Hai-xu , WANG Yang , ZHAO Yan-lin , ZHAO Jin , LIU Meng-jun.Molecular Classification of Jujube Witches' Broom(JWB) Associated Phytoplasma from Ziziphus jujuba Mill.'Zanhuangdazao'[J].Scientia Agricultura Sinica,2011,44(21):4429-4437.
Authors:YANG Hai-xu  WANG Yang  ZHAO Yan-lin  ZHAO Jin  LIU Meng-jun
Institution:YANG Hai-xu1,WANG Yang2,ZHAO Yan-lin2,ZHAO Jin2,LIU Meng-jun1(1Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding 071001,Hebei,2College of Life Science,Baoding 071000,Hebei)
Abstract: 【Objective】 Jujube Witches’ Broom (JWB) is a serious disease caused by phytoplasma. In this paper, the taxonomic status of JWB-associated phytoplasma from Ziziphus jujuba Mill. ‘Zanhuangdazao’,which is the only natural triploid cultivar in Chinese jujube, was identified.【Method】16S rDNA gene was amplified by using specific conservative primers and the nested-PCR primers, then the PCR product was cloned and sequenced. Homology analysis, dendrogram and virtual RFLP were also obtained by using related software. 【Result】 One set of the conserved primers was designed to amplify a 1 850 bp fragment (GenBank GU184180) which included 16S rDNA (1 529 bp), adjacent spacer sequence (264 bp) and partial sequence of 23S rDNA (57 bp) by PCR from total DNA extracted from diseased Ziziphus jujuba Mill. ‘Zanhuangdazao’. Nucleotide sequencing of the amplified fragment indicated that JWB-associated phytoplasma from Ziziphus jujuba Mill. ‘Zanhuangdazao’ shared nucleotide similarity about 87% to 98% with other phytoplasmas from data of GenBank, and a maximum similarity at 98% with Elm yellows(EY)phytoplasma. A phylogenetic tree based on 16S ribosomal DNA sequences was constructed. The result of virtual RFLP showed a significant difference among JWB-associated phytoplasma from Ziziphus jujuba Mill. ‘Zanhuangdazao’ and 4 subgroups. The blast result of tuf also showed that JWB-associated phytoplasma from Ziziphus jujuba Mill. ‘Zanhuangdazao’ (JN001985) shared nucleotide similarity about 52.3% to 76.7% with 4 subgroups. 【Conclusion】 JWB-associated phytoplasma from Ziziphus jujuba Mill. ‘Zanhuangdazao’ was clustered into the elm yellows group (16Sr V) phytoplasma, and it shared the highest similarity with the B subgroups. Its taxonomic status is consisting with those diploid cultivars.
Keywords:Ziziphus jujuba Mill  'Zanhuangdazao'  JWB-associated phytoplasma  16S rDNA  molecular classification  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国农业科学》浏览原始摘要信息
点击此处可从《中国农业科学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号