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猪POU1F1基因启动子区多态分析及其与生长性状的关联
引用本文:宋成义,赵芹,高波,王宵燕,吴晗,周辉云,经荣斌,毛九德.猪POU1F1基因启动子区多态分析及其与生长性状的关联[J].中国农业科学,2011,44(24):5067-5072.
作者姓名:宋成义  赵芹  高波  王宵燕  吴晗  周辉云  经荣斌  毛九德
作者单位:1.扬州大学动物科学与技术学院,中国江苏扬州 225009; 2.美国密苏里大学动物科学系,美国密苏里州哥伦比亚市 65211
基金项目:江苏省自然科学基金(BK2010317); 江苏省科技支撑计划(农业)(BE2009330-3); 江苏高校优势学科建设工程资助项目(PAPD)
摘    要: 【目的】获得猪POU1F1基因启动子区的多态信息,揭示其在不同品种中的分布特点,阐明其与生长性状的关联性。【方法】采用PCR克隆、DNA测序和PCR-RFLP技术进行POU1F1基因启动子区多态分析,利用一般线形模型分析其与生长性状的关联性。【结果】在POU1F1基因1.5 kb的启动子区域内发现5个多态位点;其中,5 bp短片段插入或缺失突变的A等位基因频率在引入品种猪中最高,在培育品种中中等,在中国地方品种最低。一般线形模型分析表明,该多态位点与猪的生长性状存在显著相关。多重比较分析发现,AA基因型个体的体高、体长、胸围和体重显著高于AB和BB基因型个体(P<0.05),而AB和BB基因型个体的体高、胸围和体重差异不显著(P>0.05),但AB基因型个体的体长显著高于BB基因型个体(P<0.05)。【结论】5 bp短片段插入或缺失突变的A等位基因是生长性状的优势等位基因,是生长性状可能的分子标记。

关 键 词:    POU1F1基因    多态    生长性状
收稿时间:2011-01-10

Polymorphism Analysis of POU1F1 Promoter Region and It's Association with Growth Traits
SONG Cheng-yi , ZHAO Qing , GAO Bo , WANG Xiao-yan , WU Han , ZHOU Hui-yun , JING Rong-bin , MAO Jiu-de.Polymorphism Analysis of POU1F1 Promoter Region and It's Association with Growth Traits[J].Scientia Agricultura Sinica,2011,44(24):5067-5072.
Authors:SONG Cheng-yi  ZHAO Qing  GAO Bo  WANG Xiao-yan  WU Han  ZHOU Hui-yun  JING Rong-bin  MAO Jiu-de
Institution:SONG Cheng-yi1,ZHAO Qing1,GAO Bo1,WANG Xiao-yan1,WU Han1,ZHOU Hui-yun1,JING Rong-bin1,MAO Jiu-de2(1College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,Jiangsu,China,2Department of Animal Sciences,University of Missouri,Columbia,MO 65211,USA)
Abstract:【Objective】 The aim of current study is to obtain the polymorphisms of POU1F1 promoter region, investigate the distribution of these polymorphisms among different pig breeds and detect it’s association with growth traits. 【Method】 PCR cloning, DNA sequencing and PCR-RFLP techniques were applied to detect the polymorphisms of POU1F1 promoter region,and the general linear model was used to analyze it’s association with growth traits. 【Result】 Five polymorphism sites were identified in the 1.5 kb POU1F1 promoter region, and the frequency of A allele in the 5 bp insertion or deletion mutation was the highest in introduced pig breeds, moderate in bred breeds, and lowest in Chinese native breeds. The general linear model analysis indicated that this polymorphism site was significantly associated with growth traits. LSD showed that the pigs with AA genotype had higher body highness, body length, circumference and body weight at 6-month-old of age compared with that of AB and BB pigs, but the difference of body highness, circumference and body weight between AB and BB pigs was not significant, while the body length of AB pigs was significantly higher than that of BB pigs. 【Conclusion】 The A allele in 5bp insertion or deletion mutation site is favorable, and it could be useful in molecular marker assisted selection for growth traits.
Keywords:pig  POU1F1 gene  polymorphism  growth traits  
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