鼠李糖乳酸杆菌对Caco-2细胞抗氧化功能的影响

 【目的】研究鼠李糖乳酸杆菌（Lactobacillus rhamnosus）对氧化应激状态下Caco-2细胞抗氧化功能的影响。【方法】将培养的Caco-2细胞分为4组：对照组和氧化应激组（在培养液中加入100 µmol?L-1 H2O2），处理组Ⅰ和处理组Ⅱ在氧化应激条件下分别添加鼠李糖乳酸杆菌（约108 CFU?mL-1）和抗氧化剂特丁基对苯二酚（TBHQ） （2.75 µg?mL-1）各1 mL。Caco-2细胞培养至12和48 h时分别测定其上清液和裂解液的抗氧化活性。【结果】添加鼠李糖乳酸杆菌减轻了Caco-2细胞氧化受损，使细胞培养上清中的总抗氧化力（T-AOC）显著高于氧化应激组：12 h时显著提高了细胞培养上清谷胱甘肽过氧化物酶（GSH-Px）（P＜0.01）、过氧化氢酶（CAT）（P＜0.01）活力以及细胞裂解液中超氧化物酶（SOD）活力（P＜0.01）和还原型谷胱甘肽（GSH）含量（P＜0.05），48 h时显著提高了细胞培养上清液抗O2- (ASAFR) （P＜0.01）、GSH-Px（P＜0.01）、CAT（P＜0.01）、SOD活力（P＜0.01）和细胞裂解液中过氧化物酶（POD）活力（P＜0.01），丙二醛（MDA）含量显著降低（P＜0.01）。添加TBHQ组12 h时细胞上清中T-AOC（P＜0.01）和CAT活性（P＜0.01）及细胞裂解液中GSH含量（P＜0.01）显著高于氧化应激组，而处理48 h后相应指标低于氧化应激组；此时，细胞培养上清液中MDA含量极显著降低（P＜0.01），抗O2-、SOD、GSH-Px、POD活力和细胞裂解液中POD活力显著升高（P＜0.05）。【结论】在体外条件下，鼠李糖乳酸杆菌可以提高氧化应激状态下Caco-2细胞的抗氧化功能。

Antioxidative Function of Lacbacillus rhamnosus to Caco-2 Cells

【Objective】This experiment was conducted to study the antioxidative function of Lactobacillus rhamnosus to Caco-2 cells under oxidative stress. 【Method】 Caco-2 cells were randomly divided into 4 groups, control group and oxidative stress group (100 µmol?L-1 H2O2), treatment groupsⅠandⅡ were added with 1 mL Lactobacillus rhamnosus (about 108 CFU?mL-1) and tertiary butylhydroquinone (TBHQ) (2.75 µg?mL-1 ) under oxidative stress, respectively. Antioxidative activities in culture supernatants and lysates of Caco-2 cells at 12 h and 48 h were measured. 【Result】 Compared to oxidative stress group, the total antioxidation capacity (T-AOC), the glutathione peroxidase (GSH-Px) (P＜0.01), catalase (CAT) (P＜0.01) activities in the cultured supernatants, and the superoxide dismutase (SOD) activity (P＜0.01), and the glutathione (GSH) content (P＜0.05) in the cells lysates at 12 h in treatment groupⅠincreased, and the increases of the antisuperoxide anion radical (O2-) (P＜0.01), GSH-Px (P＜0.01), CAT (P＜0.01), SOD (P＜0.01) activities in the cells culture supernatants and the peroxidase (POD) activity (P＜0.01) and the decreases of malondialdehyde (MDA) content (P＜0.01) in the cells lysates at 48 h were also observed. In treatment groupⅡ, the T-AOC (P＜0.01), CAT activity (P＜0.01) in the cells culture supernatants and the glutathione (GSH) content (P＜0.01) in the cells lysates at 12 h increased significantly, but these values and the MDA content at 48 h decreased significantly (P＜0.01). The anti-superoxide anion radical (O2-), SOD, GSH-Px, POD activities in the cells culture supernatants and the POD activity in the cells lysates at 48 h increased (P＜0.05).【Conclusion】These results indicated that adding Lactobacillus rhamnosus could increase the antioxidative function of Caco-2 cells under oxidative stress under in vitro conditions.