| 褪黑激素对Bcl-2和Bax基因在绒山羊皮肤中表达的影响 |
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| 引用本文： | 刘慧,张燕军,李金泉,张文广,王志新,张永斌,何云梅,蒋再平,丽春,冯林,王瑞军.褪黑激素对Bcl-2和Bax基因在绒山羊皮肤中表达的影响[J].中国农业科学,2012,45(12):2462-2467. |
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| 作者姓名： | 刘慧 张燕军 李金泉 张文广 王志新 张永斌 何云梅 蒋再平 丽春 冯林 王瑞军 |
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| 作者单位： | 1.内蒙古农业大学动物科学学院，呼和浩特 010018;
2.内蒙古农业大学动物遗传育种与繁殖重点实验室，呼和浩特 010018;
3.内蒙古白绒山羊种羊场，内蒙古鄂尔多斯 017000;
4.鄂托克旗改良站，内蒙古鄂尔多斯 016100；5鄂托克旗饲料管理站，内蒙古鄂尔多斯 016100 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(CARS-40);公益性行业(农业)科研专项(201003019);国家科技支撑计划项目(2011BAD28B05);国家自然科学基金项目(30960246) |
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| 摘 要： | 【目的】研究褪黑激素对内蒙古绒山羊皮肤Bcl-2和Bax基因表达的影响，探讨Bcl-2和Bax基因以及褪黑激素与绒山羊绒毛生长和凋亡的关系。【方法】选用8只性别、体重、年龄一致的内蒙古绒山羊母羊，随机分为埋植组和对照组，提取皮肤总RNA，对Bcl-2和Bax基因mRNA表达进行相对定量分析。【结果】①Bcl-2和Bax基因在内蒙古绒山羊皮肤细胞中相对表达量比值（Bcl-2/Bax）的增减与绒山羊一年中绒毛生长、退行以及休止时间特点基本吻合；②褪黑激素对Bcl-2基因在内蒙古绒山羊皮肤中的表达无显著影响（P＞0.05），但显著上调了Bax基因在各月份的表达（P＜0.01）。【结论】褪黑激素显著下调了Bcl-2和Bax基因在内蒙古绒山羊皮肤细胞中相对表达量的比值（Bcl-2/Bax），可促进细胞凋亡，加快机体新陈代谢。
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| 关 键 词： | 绒山羊 褪黑激素 Bcl-2基因 Bax基因 |
| 收稿时间： | 2011-08-17 |
Effects of Melatonin on the Expression of Bcl-2 and Bax Genes in Skin of Cashmere Goat |
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| LIU Hui,ZHANG Yan-jun,LI Jin-quan,ZHANG Wen-guang,WANG Zhi-xin,ZHANG Yong-bin,HE Yun-mei,JIANG Zai-ping,LI Chun,FENG Lin,WANG Rui-jun.Effects of Melatonin on the Expression of Bcl-2 and Bax Genes in Skin of Cashmere Goat[J].Scientia Agricultura Sinica,2012,45(12):2462-2467. |
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| Authors: | LIU Hui ZHANG Yan-jun LI Jin-quan ZHANG Wen-guang WANG Zhi-xin ZHANG Yong-bin HE Yun-mei JIANG Zai-ping LI Chun FENG Lin WANG Rui-jun |
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| Institution: | 1,2(1College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018;2Animal Genetics,Breeding and Reproduction Key Laboratory of Inner Mongolia Agricultural University,Hohhot 010018;3Inner Mongolia White Cashmere Goat Sheepstud,Ordos 017000,Inner Mongolia;4Etuoke Improving Station,Ordos 016100,Inner Mongolia;5Etuoke Feed Management Station,Ordos 016100,Inner Mongolia) |
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| Abstract: | 【Objective】The present study was aimed to research the effects of melatonin on the expression of Bcl-2 and Bax genes in the skin of Inner Mongolia Cashmere goat,then discuss the relationships between Bcl-2,Bax,and melatonin and the growth and apoptosis of cashmere goat.【Method】 Eight Inner Mongolia Cashmere goats,with the same age,sex and liveweight were chosen.Four of them were treated with melatonin,the rest were used as control.Total RNA of skin was extracted,and expression of Bcl-2 and Bax genes were analyzed by real-time PCR.【Result】 The changing characteristics of Bcl-2/Bax coincided with the growth rhythm of cashmere of Inner Mongolia Cashmere goat.Melatonin had no significant effects on expression of Bcl-2 gene(P>0.05),but significantly up-regulated the Bax gene(P<0.01).【Conclusion】 Melatonin down-regulated the ratio of Bcl-2/Bax in the skin of Inner Mongolia Cashmere goat,which promoted the apoptosis and accelerated the metabolism. |
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| Keywords: | cashmere goat melatonin Bcl-2 gene Bax gene |
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