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转NDR1基因烟草对赤星病和晚疫病的抗性增强
引用本文:窦道龙,王冰山,朱生伟,唐益雄,王志兴,孙敬三,李仁敬,张振南.转NDR1基因烟草对赤星病和晚疫病的抗性增强[J].中国农业科学,2003,36(10):1120-1124.
作者姓名:窦道龙  王冰山  朱生伟  唐益雄  王志兴  孙敬三  李仁敬  张振南
作者单位:1. 中国科学院植物研究所,北京,100093;中国农业科学院生物技术研究所,北京,100081
2. 中国农业科学院生物技术研究所,北京,100081
3. 中国科学院植物研究所,北京,100093
4. 新疆中国彩棉股份有限公司,乌鲁木齐,830016
基金项目:国家自然科学基金(30170637),国家转基因研究与产业化专项资助项目(ZJY-A-01)
摘    要: NDR1(nonrace-specific disease resistance)基因在植物系统获得性抗性的信号传导途径中起着重要的作用,过量表达该基因的拟南芥对不同病原菌的抗性都有提高。利用PCR技术首次克隆了拟南芥Wassilewskija生态型的NDR1基因,与Columbia生态型相比,该基因共有7处碱基不同,引起编码氨基酸变化4处。构建了植物高效表达载体,利用农杆菌介导法转化烟草,经PCR和Southern鉴定,外源基因已整合到植物基因组中。随机挑选10个转基因株系进行抗病性分析,其中3个株系对赤星病和晚疫病的抗性明显增强。

关 键 词:烟草  NDRI基因  广谱抗病基因工程  赤星病  晚疫病
修稿时间:2002年5月13日

Transgenic Tobacco with NDR1 Gene Improved Its Resistance to Two Fungal Diseases
DOU Dao-long,WANG Bing-shan,ZHU Sheng-wei,TANG Yi-xiong,WANG Zhi-xing,SUN Jing-san,LI Ren-jing,ZHANG Zhen-nan.Transgenic Tobacco with NDR1 Gene Improved Its Resistance to Two Fungal Diseases[J].Scientia Agricultura Sinica,2003,36(10):1120-1124.
Authors:DOU Dao-long  WANG Bing-shan  ZHU Sheng-wei  TANG Yi-xiong  WANG Zhi-xing  SUN Jing-san  LI Ren-jing  ZHANG Zhen-nan
Abstract:The NDRl (nonrace-specific disease resistance) gene is required in Arabidopsis resistance to both bacterial and fungal pathogen mediated by several R genes. Expression of NDRl in transgenic plants confers resistance to a broad variety of plant pathogens. The NDRl gene from Arabidopsis Wassilewskija cultivation was cloned. In contract to the gene of Columbia cultivation, this gene has 7 nucleic acids and 4 amino acids mutations, respectively. A plant high effective expression vector was constructed and transformed to tobacco by agrobacterial-mediated transformation. Analysis of PCR and southern blot showed that NDRl gene has been integrated in tobacco genome. Three of ten transgenic plants selected randomly have obviously improved its resistance to Altemaria alternata and Phytophthora infestans.
Keywords:Nicotiana tabacum  NDR1 gene  Genetically engineering for plant broad-spectrum disease resistance  Alternaria alternata  Phytophthora infestans
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