| 蜜蜂(Apis mellifera)腺苷酸转移载体基因cDNA的电子克隆 |
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| 引用本文： | 陈大福,牛宝龙,翁宏飚,孟智启,吕顺霖.蜜蜂(Apis mellifera)腺苷酸转移载体基因cDNA的电子克隆[J].中国农业科学,2004,37(6):923-927. |
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| 作者姓名： | 陈大福 牛宝龙 翁宏飚 孟智启 吕顺霖 |
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| 作者单位： | [1]浙江大学动物科学学院，杭州310029 [2]浙江省农业科学院蚕桑研究所，杭州310021 |
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| 摘 要： | 以果蝇腺苷酸转移载体基因cDNA序列为信息探针,对蜜蜂EST数据库进行同源检索筛选,克隆了蜜蜂腺苷酸转移载体基因(Am ant)的cDNA序列(GenBank登记号为AY332626),该基因全长1 251bp。经RT-PCR克隆、序列分析验证,结果表明与电子克隆序列完全一致;该基因具有完整的开放阅读框架(ORF),编码蛋白为300个氨基酸,通过对人、果蝇、家蚕及烟草天蛾的腺苷酸转移载体蛋白序列比较,发现该基因具有高度的保守性;说明根据物种间同源基因序列,进行跨物种EST数据库的同源检索筛选、拼接,是基因克
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| 关 键 词： | 电子克隆 EST数据库 RT-PCR 腺苷酸转移载体 蜜蜂 |
In Silico Cloning of Honeybee (Apis mellifera) Adenine Nucleotide Translocator Gene cDNA |
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| Abstract: | A novel honeybee adenine nucleotide translocator gene (Am ant) cDNA (GenBank Accession, AY332626) was cloned by blasting the honeybee EST database with the fruit fly homologous ant gene cDNA sequence information as a querying probe. It was also verified by RT-PCR. The full-length Am ant cDNA of 1251bp contains a complete ORF encoding adenine nucleotide translocator (ANT), which was very conservative in human, fruit fly, silkworm and tobacco hornworm. The results show that it is a convenient technique for cloning new gene by searching EST database with homologous gene of model species such as fruit fly. |
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| Keywords: | In silico cloning EST database RT-PCR Adenine nucleotide translocator Honeybee |
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