河北地区花生根瘤菌的系统发育多样性研究

 【目的】了解中国北方地区花生根瘤菌的多样性状况。【方法】采用16S rDNA PCR-RFLP、16S～23S IGS PCR-RFLP和16S rRNA基因的序列分析方法对分离自河北地区的58株花生根瘤菌以及Rhizobium、Agrobacterium、Sinorhizobium、Mesorhizobium和Bradyrhizobium的参比菌株进行系统发育多样性比较分析。【结果】16S rDNA PCR-RFLP方法在细菌属水平上聚群良好；而16S～23S IGS PCR-RFLP则适于属和种水平上的聚群。3种方法在系统发育上得到基本一致的结果。其中有38株花生根瘤菌是与Bradyrhizobium关系密切的慢生菌株，并且与B. japonicum和B. liaoningense的关系最为接近，与有关中国西南地区和华中地区花生根瘤菌的报道结果相似。然而供试菌株中还存在6株快生和10株中慢生花生根瘤菌，其中快生菌在系统发育关系上接近R. yanglingense、R. mongolense和R. gallicum。【结论】河北地区的花生根瘤菌具有更大的多样性。

Phylogenetic Diversity of Rhizobia Isolated from the Root Nodules of Peanut (Arachis hypogaea) Grown in Hebei

Objective ] We hope to understand the diverse state of peanut rhizobia in northern part of China. Method ] Fifty eight rhizobial strains isolated from root nodules of peanut plants grown at different sites in Hebei Province were analyzed and compared with the reference strains of the species of Rhizobium, Agrobacterium, Sinorhizobium, Mesorhizobium and Bradyrhizobium by 16S rDNA PCR-RFLP, 16S-23S IGS PCR-RFLP and 16S rRNA gene sequencing. Results] The results showed that thirty eight strains belonged Bradyrhizobium related to Bradyrhizobiumjaponicum and Bradyrhizobium liaoningense. Conclusion] It demonstrates that peanut plants nodulate mainly with Bradyrhizobium strains in Hebei, similar to the previous reports with rhizobia from peanut plants grown in other regions of China. However, the existence of six fast-growing rhizobial strains related to R. yanglingense, R. mongolense, and R. gallicum and of ten moderately slow-growing strains indicate that peanut plants attract diverse rhizobia in Hebei Province.