植物乳杆菌C88对H2O2诱导氧化损伤的 Caco-2细胞的保护作用

【目的】文章旨在研究植物乳杆菌（Lactobacillus plantarum）C88的抗氧化作用。【方法】采用0.1 mmol?L-1 和0.2 mmol?L-1浓度H2O2连续处理Caco-2细胞12—48 h造成氧化损伤，通过测定细胞培养液上清和细胞裂解液的自由基清除能力及抗氧化物酶活性，评价植物乳杆菌C88对Caco-2细胞抗氧化损伤的保护作用。【结果】与氧化损伤模型组相比，植物乳杆菌C88在1011CFU/mL浓度下，能显著提高细胞裂解液的谷胱甘肽过氧化物酶（GSH-Px）活性(P＜0.05)和总抗氧化（T-AOC）能力(P＜0.05)；以及细胞裂解液的羟自由基清除率(P＜0.05)、谷胱甘肽过氧化物酶（GSH-Px）(P＜0.05)和超氧化歧化酶（SOD）活性。细胞裂解液的羟自由基清除率低于氧化应激对照组。【结论】植物乳杆菌（L. plantarum）C88通过提高Caco-2细胞的自由基清除能力和抗氧化酶活性，表现出了较强的抗氧化活性。

Protective Effect of Lactobacillus plantarum C88 on H2O2-Induced Oxidative Stress in Caco-2 Cells

【Objective】 The aim of the study was to evaluate the antioxidant activity of L. plantarum C88 against H2O2 induced Caco-2 cell oxidative stress.【Method】In vitro methods simulating gastric and small intestine digestive processes, coupled with human colon carcinoma cells in oxidative stress, to hydrogen peroxide at concentrations of 0.1 mmol?L-1 and 0.2 mmol?L-1 for 12 h, 24 h, 36 h, 48 h, are the valid tools to evaluate the antioxidant activity of L. plantarum C88. The free radical scavenging capacity and antioxidant enzymes activitties in the cell culture supernatants and cell disruptions were measured.【Result】Compared with the oxidative stress group, L. plantarum C88 at 1011CFU/mL signi?cantly increased the glutathione peroxidase (GSH-Px) (P＜0.05) and the total antioxidation capacity (T-AOC) (P＜0.05) in the cultured supernatants, the hydroxyl radical scavenging ability (P＜0.05), the glutathione peroxidase (GSH Px) (P＜0.05) and the superoxide dismutase (SOD) activity in the cell disruptions.【Conclusion】Results of the study suggested that L. plantarum C88 could protect the Caco-2 cells against H2O2-induced oxidative stress by renewing the enzymatic and non-enzymatic antioxidant defense system.