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槲皮素对肉鸡脂肪细胞环腺苷酸信号通路的作用
引用本文:欧阳文文,李垚,赵伟,王明昊,金芳.槲皮素对肉鸡脂肪细胞环腺苷酸信号通路的作用[J].中国农业科学,2013,46(13):2769-2776.
作者姓名:欧阳文文  李垚  赵伟  王明昊  金芳
作者单位:东北农业大学动物营养研究所,哈尔滨 150030
基金项目:国家科技支撑计划项目(2006BAD14B02-8-1)、哈尔滨市科学技术局科技创新人才研究专项资金(2011RFLXN015)
摘    要:【目的】研究槲皮素对肉鸡脂肪细胞甘油三酯沉积过程中环腺苷酸(cyclic adenosine monophosphate,cAMP)信号通路的作用。【方法】将脂肪细胞随机分5组,每组6个重复;空白对照组为基础培养液,溶剂对照组为含2‰二甲基亚砜(DMSO)的培养液,试验组为含10、20和40 mg?L-1槲皮素的培养液,分别于24、48和72 h收集细胞及培养液;ELISA法测定乙酰CoA羧化酶(acetyl CoA carboxylase,ACC)、脂肪酸合成酶(fatty acid synthase,FAS)、脂蛋白脂酶(lipoprotein lipase,LPL)、cAMP、腺苷酸环化酶(adenylate cyclase,AC)、磷酸二脂酶(phosphodiesterase,PDE)、蛋白激酶(protein kinase A,PKA)含量;紫外分光光度计测定甘油三酯(triacylglycerides,TG)含量。【结果】与空白对照组相比,①溶剂对照组各指标均无显著差异(P>0.05);②20和40 mg?L-1槲皮素组ACC、FAS、LPL、TG和PDE含量显著降低(P<0.05),cAMP和PKA含量显著增加(P<0.05);③3个槲皮素组AC含量均无显著差异(P>0.05)。【结论】一定剂量槲皮素可通过调控cAMP信号通路抑制脂肪合成,并减少脂肪沉积。

关 键 词:槲皮素    脂肪细胞    脂肪代谢    环腺苷酸    信号通路
收稿时间:2012-12-24

Effect of Quercetin on cAMP Signaling Pathway in Chicken Adipocytes
Institution:Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030
Abstract:【Objective】This experiment was conducted to study the effects of quercetin on cAMP signaling pathway in broilers adipocytes. 【Method】 Adipocytes were randomly allocated into 5 groups with 6 replicates per group. Adipocytes were cultured in basal medium, 2‰ DMSO medium or addition of 10, 20, 40 mg•L-1 quercetin medium for 24 h, 48 h and 72 h, respectively. Then adipocytes and medium were collected for measuring the contents of ACC, FAS, LPL, cAMP, AC, PDE, PKA by ELISA and TG by ultraviolet spectrophotometer, respectively.【Result】Compared with the control, all of these indicators of adipocytes were not significantly affected by 2‰ DMSO (P>0.05). The contents of ACC, FAS, LPL,TG, and PDE were significantly decreased in medium supplemented with 20 mg•L-1 and 40 mg•L-1 quercetin (P<0.05), cAMP and PKA contents were significantly increased (P<0.05); Intracellular AC content was not significantly affected by quercetin (P>0.05).【Conclusion】The results indicated that quercetin inhibited lipogenesis and fat deposition via regulating cAMP signaling pathway.
Keywords:quercetin  adipocytes  lipometabolism  cAMP  signaling pathway
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