烟碱对B型和Q型烟粉虱取食行为的影响——基于EPG和液体饲囊技术体系

【目的】明确烟碱对Ｂ型和Ｑ型烟粉虱取食行为的影响，并据此建立一套研究次生物质对烟粉虱取食行为影响的利用刺吸电位（electrical penetration graph，EPG）结合液体饲囊（liquid diet sac，LDS）（EPG+LDS）指标体系。【方法】利用EPG技术测定烟粉虱对不同成分LDS的取食行为，定量研究不同浓度烟碱对烟粉虱取食行为的影响。【结果】利用植物作对照，比较5%、10%和15%蔗糖溶液对烟粉虱取食波形的影响，结果表明10%蔗糖浓度下烟粉虱EPG波形的相对振幅、频率与其在植物上的行为最为相近。在10%蔗糖溶液中加入烟碱，随着烟碱浓度的升高，烟粉虱取食波形的持续时间呈现逐渐减少的趋势，且0.001%的烟碱浓度即可显著延长烟粉虱的刺探时间并显著抑制E1波和E2波的持续时间。高浓度烟碱条件下，E2波被完全抑制。虽然B型和Q型烟粉虱在多个刺探过程的EPG指标上差异不显著，但与吸食相关的几个指标差异显著，Q型烟粉虱E2波持续时间无论在无烟碱环境下还是较低烟碱环境下都显著长于B型烟粉虱。【结论】在EPG波形指标上，C波持续总时间、E1波持续总时间及E2波持续总时间等是EPG+LDS技术体系较为重要的行为指标；对于LDS指标，10%的蔗糖溶液更适合用于不同生物型烟粉虱取食行为的EPG研究；烟碱明显抑制烟粉虱2种生物型的取食行为，Q型烟粉虱对烟碱具有更强的适应能力。

Effects of Nicotine on Feeding Behavior of Bemisia tabaci B and Q Biotypes Based on EPG and Liquid Diet Sac Technique

【Objective】 The effects of nicotine on the feeding behaviors of the Bemisia tabaci B and Q biotypes were investigated, with the aim of establishing a corresponding evaluating system based on the electrical penetration graph (EPG) and the liquid diet sac (LDS) technique. 【Method】 EPG method combined with a LDS was used to quantify the effect of the concentrations of nicotine on the feeding behaviors of the B. tabaci. 【Result】 Relative amplitudes and frequencies in EPG waveforms of the B. tabaci in 10% sucrose solution, compared to those in 5% and 15% sucrose solutions, were analogous to the waveforms of B. tabaci on plants. The durations of ingestion waveforms decreased with the increase of nicotine levels in 10% sucrose solution. Salivation (E1) and passive ingestion (E2) durations were reduced and the penetration duration was prolonged even at the low concentration (0.001%) of nicotine. The passive ingestion from LDS was completely inhibited at a higher concentration of nicotine (0.01%). Although the major EPG pathway variables between B. tabaci B and Q biotypes were similar, the duration of passive ingestion of Q biotype was significantly longer than that of the B biotype on the diet without nicotine or with lower nicotine concentration. 【Conclusion】 The valuable EPG variables for EPG+LDS system include the durations of C, E1 and E2 patterns. Ten percent sucrose solution in LDS was more suitable for recording the feeding behaviors of B. tabaci using EPG technique. The feeding behaviors of B. tabaci B and Q biotypes were signi?cantly inhibited by nicotine, but Q biotype had higher nicotine adaptability than B biotype.