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解偶联剂DNP处理对采后龙眼果实果皮褐变和活性氧代谢的影响
引用本文:陈莲,陈梦茵,林河通,陈艺晖,林艺芬,陈绍军.解偶联剂DNP处理对采后龙眼果实果皮褐变和活性氧代谢的影响[J].中国农业科学,2009,42(11):4019-4026.
作者姓名:陈莲  陈梦茵  林河通  陈艺晖  林艺芬  陈绍军
作者单位: 
基金项目:国家自然科学基金,国家科技支撑计划专项,福建省自然科学基金,福建省重点科技项目,国家大学生创新性实验计划 
摘    要: 【目的】研究呼吸解偶联剂2,4-二硝基苯酚(DNP)对采后龙眼果实果皮褐变和活性氧代谢的影响。【方法】采后龙眼果实用0.1 mmol?L-1的DNP浸泡 0.5 h,以蒸馏水处理的果实为对照,果实晾干后用0.015 mm厚的聚乙烯薄膜袋密封包装,在(28?1)℃下贮藏。定期测定贮藏期间果皮褐变指数、三磷酸腺苷(ATP)和丙二醛(MDA)含量、超氧自由基( )产生速率、活性氧清除酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)]活性、内源抗氧化物质还原型谷胱甘肽(GSH)和还原型抗坏血酸(AsA)]含量的变化。【结果】与对照果实相比,经DNP处理的龙眼果实果皮褐变指数增大,果皮ATP含量降低。同时,DNP处理促进龙眼果皮产生 的速率增加,且在整个贮藏期间维持较高水平。APX活性下降,SOD和CAT活性上升,内源抗氧化物质GSH和AsA含量明显下降,MDA含量增加。【结论】DNP促进龙眼果皮褐变可能是由于DNP降低活性氧清除能力、导致活性氧积累而破坏细胞膜系统,同时DNP导致的能量亏缺使细胞膜系统的损伤修复能力下降,其结果破坏细胞膜结构,使酚酶(多酚氧化酶,PPO)与酚类物质接触、酚类物质氧化而形成褐变聚合体的结果。

关 键 词:龙眼  果皮  褐变  活性氧代谢  能量亏缺  ATP  DNP
收稿时间:2009-01-12;

Effects of Uncouple Agent DNP Treatment on Browning and Active Oxygen Metabolism in Pericarp of Harvested Longan Fruit
CHEN Lian,CHEN Meng-yin,LIN He-tong,CHEN Yi-hui,LIN Yi-fen,CHEN Shao-jun
.Effects of Uncouple Agent DNP Treatment on Browning and Active Oxygen Metabolism in Pericarp of Harvested Longan Fruit[J].Scientia Agricultura Sinica,2009,42(11):4019-4026.
Authors:CHEN Lian  CHEN Meng-yin  LIN He-tong  CHEN Yi-hui  LIN Yi-fen  CHEN Shao-jun
Institution:(College of Food Science, Fujian Agriculture and Forestry University)
Abstract:Objective] Effects of DNP (2,4-dinitrophenol), the uncouple agent for respiratory, on browning and active oxygen metabolism in pericarp of harvested longan fruits were investigated in this paper. Method] The harvested Iongan fruits were dipped in 0.1 mmol·L~(-1) DNP for 0.5 h, while, the control fruits were dipped in distilled water. The fruits were dried prior to packaging and stored at (28±1)℃. During fruit storage, pericarp browning index, contents of adenosine triphosphate (ATP) and malondialdehyde (MDA), O_2~- production rate, activities of active oxygen scavenging enzymes such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), contents of endogenous antioxidant substances such as ascorbic acid (AsA) and glutathione (GSH) were determined. Result] As compared with the control fruits, there were higher browning index and lower ATP content in pericarp of DNP-treated longan fruits. The longan fruits treated with DNP resulted in increased rate of O_2~- production and kept higher rate of O_2~- production during the whole storage, reduced the activity of APX and increased the activities of SOD and CAT, significantly decreased the amounts of AsA and GSH, and increased MDA content. Conclusion] From the results it can be concluded that DNP-induced browning of longan pericarp may be due to the decrease of active-oxygen-scavenging capacity and the accumulation of active oxygen caused by DNP treatment, which may destroy cell membrane system. And limited energy availability caused by DNP may lead to weaken repair capacity of the damage of cell membrane system. All these factors may disrupt cellular membrane structure, allowing phenolase (polyphenol oxidase, PPO) to react with phenolic substrates and oxidation phenolics to form brown polymers.
Keywords:ATP  DNP  longan (Dimocarpus longan Lour  )  pericarp  browning  active oxygen metabolism  limited energy availability  ATP  DNP
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