特征碳源简化土壤微生物Biolog测定的方法及应用

【目的】从发生番茄青枯病的土壤微生物群落Biolog测定结果中,在ECO微平板10种特征碳源基础上,减为9种特征碳源,利用MT板进行试验,以简化其测定手续。【方法】选用盆栽番茄土样进行了特征碳源的浓度试验,并应用所确定的浓度分别针对施肥处理不同但均未发病的大田辣椒土样、发病程度不同的大田番茄土样和盆栽辣椒土样进行了3次验证试验。【结果】浓度试验结果表明,各碳源不同浓度的OD值普遍存在差异,将其与ECO板结果相关联后确定了各特征碳源的应用浓度。3次验证试验结果表明,选用的试验浓度能明显地区分作物发病差异。【结论】特征碳源能反映出茄科作物病土与健康土以及发病轻与重的区别,可以简化土壤微生物Biolog测定。

Method for Simplification of Characteristic Carbon Sources for Biolog Analysis of Soil Microbial Community and Its Application

Objective In order to simplify the Biolog analysis, 9 kinds of characteristic carbon sources selected from the carbon resources in Biolog ECO microplate containing 10 carbon resources and added in Biolog MT microplate to analyse soil microbial cummunity where bacterial wilt of tomato occurred. Method The optimal concentration of characteristic carbon resources added in Biolog MT microplates was studied with the soil of potted tomato and three tests of verifying the carbon resource concentrations were carrie...