运输前后奶牛血清蛋白质组的变化

【目的】从血清蛋白组研究运输对奶牛机体的影响，充分理解运输对奶牛的生理病理影响及其潜在的作用机制提供理论依据。【方法】采用二维凝胶电泳（2-DE）结合MADIL-TOF-TOF串联质谱的方法对运输前第7天、运输后3 h和第7天奶牛的血清进行蛋白质组分析鉴定。【结果】运输可引起奶牛血清中14个蛋白点的表达丰度发生变化，有12个蛋白点得到有效鉴定。与运输前第7天相比，血清白蛋白、IgG1 重链恒定区、类型II细胞骨架I和转甲状腺素蛋白在运输后3 h表达量降低，而α1-酸性糖蛋白、结合珠蛋白、nesprin-2、微管微丝交联因子1、酪氨酸蛋白激酶Fps85D、输出蛋白5和一个未知蛋白点在运输后3 h的表达量升高；在运输后第7天，除α1 酸性糖蛋白外，上述蛋白的表达量与运输前第7天无显著差异。【结论】运输引起变化的蛋白主要涉及机体急性期应答和免疫反应、物质运输以及细胞内多个代谢途径，表明运输引起了奶牛应激。

Effects of Transportation on Serum Proteome in Dairy Cows

【Objective】The experiment was designed to investigate the effects of transport on serum proteome of dairy cows.【Method】Proteome analysis in serum from dairy cows on the 7 d before transport,at 3 h and on the 7 d after transport were performed using two-dimensional electrophoresis(2-DE) coupled with MADIL-TOF-TOF mass spectrum.【Result】Fourteen protein spots having significantly different expression levels after transport were observed.Serum albumin,Ig gamma 1 heavy chain constant region,type II cytoskeletal 1 and transthyretin were down-regulated at 3 h after transport,while alpha 1-acid glycoprotein,haptoglobin,nesprin-2-like,microtubule-actin cross-linking factor 1,tyrosine-protein kinase Fps85D,exportin 5 and an unknown protein spot were up-regulated comparative with the 7 d pre-transport.With the exception of alpha 1-acid glycoprotein,the expression volume of these proteins on the 7 d after transport were not different with those on the 7 d before transport.【Conclusion】These proteins are involved in acute phase response,immune response,transport as well as various metabolic pathways in cells,suggesting transport resulted in stress.