百脉根表达H5N1亚型禽流感血凝素的研究

 【目的】将H5N1亚型禽流感病毒血凝素（AIVHA）基因转入牧草植物中，利用豆科牧草作为植物生物反应器来生产禽流感抗原蛋白，探索研制禽流感转基因植物可饲用疫苗的可行性。【方法】以百脉根子叶柄外植体作为转化受体，通过农杆菌介导法将AIVHA基因导入百脉根，子叶柄外植体经过共培养、筛选分化、再生, 得到抗性植株。对抗性植株进行了PCR、RT-PCR检测和Western blot分析。【结果】证明AIVHA基因已经导入到百脉根基因组中，在核酸水平和蛋白水平都得到了表达。【结论】利用百脉根表达禽流感抗原蛋白是可行的。

Study on Exlpression of Avian Influenza Virus Hemagglutinin (H5N1)Gene in Lotus corniculatus

【Objiective】Lotus corniculatus is a kind of legume forage with good property. Avian influenza virus (AIV) hemagglutinin (H5N1) gene is transformed into the Lotus corniculatus in order to get the H5N1 antigen protein in it taken as the plant bioreactor. It will be helpful to manufacture the edible transgenic plant vaccine of AIV. It also has an important significance on spreading of AIV in wild migratory birds. 【Method】Taken its cotyledon petiole explant as the transformation receptors, we transform hemagglutinin gene of Avian Influenza to Lotus corniculatus by Agrobacterium-mediated transformation. After co-culture, selective differentiation and regeneration, we can get the resistant plant. Resistant plants were detected by PCR, RT-PCR and western blotting.【Result】The results prove that hemagglutinin gene has been transformed to the genome of Lotus corniculatus. Western blot analysis confirmed that hemagglutinin gene protein has been expressed on protein level in the transgenic plants and the result is better than the control.【Conclusion】It is feasible to use plant bioreactor to produce AIV H5N1 antigen protein in the legume forage.