甘蓝型油菜种皮色泽形成与相关酶及蛋白质含量的关系

 以3对不同来源的甘蓝型油菜黄、黑籽近等基因系为试材,研究了甘蓝型黄籽油菜种皮色泽形成与相关酶及蛋白质含量的关系。结果表明,相同遗传背景的黑籽种皮中,苯丙氨酸解氨酶(PAL) 和多酚氧化酶(PPO)均显著高于黄籽种皮,且黄籽种皮的PAL活性高峰期出现较晚,而谷氨酰胺合成酶(GS)活性在黄籽和黑籽种皮中无显著差异。在种子发育期用红光、蓝光、PAL抑制剂对羟基苯甲酸、PPO抑制剂聚乙烯吡咯烷酮、蛋白质合成促进剂尿素、质体蛋白质合成抑制剂氯霉素分别处理植株,发现PAL是黄籽种皮呈色的关键因素;PPO是黑籽种皮呈色的

Relationship of the Color Formation and Related Enzymes and Protein Content of the Spermoderms in Brassica napus

To study the relationship of the color formation and related enzymes and protein content of the spermodermsin Brassica napus, three pairs of different genetic near-isogenic lines of black-seed and yellow-seed Brassica napus wereapplied as the experimental materials in the research. The results showed that the activities of phenylalanine ammonia-lyase(PAL) and polyphenol oxidase(PPO) in black-seed lines were much higher than those in yellow ones under the same geneticconditions. Additionally, the peak of the PAL in yellow-seed spermoderms appeared comparatively late. However, theactivities of glutamine synthetase (GS) in yellow-seed and black-seed spermoderms presented no apparent difference.During the seeds developing, the plants of yellow-seed and black-seed Brassica napus were treated with red light, bluelight, p-thydroxybenzoic acid PAL-inhibiter, polyvinylpyrrolidone PPO-inhibiter, urea the promoter of protein synthesisand chloramphenicol the inhibiter of plastid protein synthesis, respectively. It was found that PAL and PPO might be the keyfactors to make the yellow-seed and black-seed spermoderms color respectively. Nitrogen assimilation might have no directrelation with the difference of the protein content of yellow-seed and black-seed spermoderms. PAL was the essential factorto make the protein content of yellow-seed and black-seed spermoderms different.