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中国西南地区芥菜型油菜资源遗传多样性分析
引用本文:蒲晓斌,王茂林,栾丽,王湘君,张锦芳,李浩杰,张启行,李熠毅,曹毅,蒋梁材,赵云.中国西南地区芥菜型油菜资源遗传多样性分析[J].中国农业科学,2007,40(8):1610-1621.
作者姓名:蒲晓斌  王茂林  栾丽  王湘君  张锦芳  李浩杰  张启行  李熠毅  曹毅  蒋梁材  赵云
作者单位:1. 四川大学生命科学学院,成都,610064;四川省农业科学院作物研究所,成都,610066
2. 四川大学生命科学学院,成都,610064
3. 中国科学院生物研究所,成都,610064
4. 四川省农业科学院作物研究所,成都,610066
基金项目:国家高技术研究发展计划(863计划);国家自然科学基金;农业部重点项目;四川省品资攻关研究项目;四川省应用基础研究计划
摘    要: 【目的】研究中国西南地区丰富的芥菜型油菜资源遗传多样性,为芥菜型油菜资源的保护和育种利用提供有益的数据。【方法】选取以西南地区为主的73份芥菜型油菜资源,分别进行14个随机引物RAPD标记多态性分析结果的聚类分析和15个主要植物学性状量测数据的聚类分析。【结果】RAPD标记多态性分析结果的聚类分析显示为3大类,共15个亚类:四川盆地资源为第1大类,含川东、川南、川北和重庆来源等6个亚类;盆周及盆周高原和云南部分资源为第2大类,分川东南、川西南和川西北来源等4个亚类;云贵高原及长江两岸为第3大类,以贵州、云南来源的资源为主,分贵阳周边、金沙江流域和云南中部来源等5个亚类。植物学性状量测数据的聚类分析显示为4大类,共14个亚类:第1大类以川东南、重庆和云南来源的资源为主,分3个亚类;第2大类以川南、川北、云南和贵州来源的资源为主,分4个亚类;第3大类以贵州、四川来源的资源为主,分4个亚类;第4大类以川西北来源的资源为主,含3个亚类。【结论】西南地区芥菜型油菜资源间差异明显,具有丰富的遗传多样性;RAPD标记多态性分析结果和植物学性状量测数据的聚类分析结果,显示分类主要遵循地域和生态环境规律,可分清芥菜型油菜资源间的亲缘关系和遗传距离远近;在分子水平上,分类结果可能更深入和准确可靠。

关 键 词:芥菜型油菜  地方资源  遗传多样性  RAPD标记  植物学性状  聚类分析
收稿时间:2006-5-31
修稿时间:2006-05-31

Genetic Diversity Analysis of Brassica juncea Landraces in Southwest China
PU Xiao-bin,WANG Mao-lin,LUAN Li,WANG Xiang-jun,ZHANG Jin-fang,LI Hao-jie,ZHANG Qi-xing,LI Yi-yi,CAO Yi,JIANG Liang-cai,ZHAO Yun.Genetic Diversity Analysis of Brassica juncea Landraces in Southwest China[J].Scientia Agricultura Sinica,2007,40(8):1610-1621.
Authors:PU Xiao-bin  WANG Mao-lin  LUAN Li  WANG Xiang-jun  ZHANG Jin-fang  LI Hao-jie  ZHANG Qi-xing  LI Yi-yi  CAO Yi  JIANG Liang-cai  ZHAO Yun
Institution:1.College of Life Science, Sichuan University, Chengdu 610064; 2.Crop Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066; 3Biology Institute, China Academy of Science, Chengdu 610064
Abstract:【Objective】Rapeseed(B.juncea L.) local germplasm resources would be conserved and utilized in rapeseed breeding more effectively, the genetic diversity in them was studied on molecular level instead of apparent genetics level. 【Method】73 resources in B.juncea L. from Sichuan or other regions were selected, the genetic relationship among them was studied using RAPD marker cluster analyzing and botanical trait cluster analyzing. 119 RAPD primers were used and 25 main botanical traits or 88 trait grades genetic stably were observed.【Results】The tested resources were divided into three groups and 13 units in the RAPD marker cluster analyzing. Qiubeiheiyoucai from Yunnan was the first group, more likely a special original resource. The majority resources in the second group were come from Yunnan. The majority resources in the third group were come from Sichuan(including Chongqing). The second group was divided into two sub-groups. The majority resources in one were come from Guizhou and the majority resources in the other were come from Guizhou and Yunnan. The third group were also divided into two sub-groups. The majority resources in one were come from the regions out of Sichuan and the majority resources in the other were come from Sichuan. The tested resources were divided into six groups and 28 units in the botanical trait cluster analyzing. The resources in the first group were come from Sichuan(including Chongqing). The resources in the second group were come from Sichuan and Guizhou. The majority resources in the third group were come from Yunnan-Guizhou plateau and Qinghai-Xizang plateau. The majority resources in the fourth group were come from Liangshan region in Sichuan and Yunnan-Guizhou plateau. B.carinata L. come from Ethiopia and Changningbendihuangyoucai come from Sichuan were the fifth group. The majority resources in the sixth group were come from Eastern Sichuan, Liangshan region in Sichuan and Yunnan-Guizhou plateau.【Conclusion】The different between resources in B.juncea L. from Sichuan and from Yunnan,Guizhou and other regions was obvious. There was abundant genetic diversity in resources in B.juncea L. from Sichuan. The genetic relationship among resources from different ecological regions or resources from the same ecological regions was near or far, decided by the specific ecological environment and the behavior of human. The genetic relationship and the genetic distance could be identified by RAPD marker cluster analyzing or botanical trait cluster analyzing. But the results from RAPD marker cluster analyzing were more reliable.
Keywords:Brassica juncea L    Landrace  Genetic diversity  RAPD marker  Agronomic trait  Cluster analysis
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