应用Charleston×东农594重组自交系群体构建SSR大豆遗传图谱

 以美国半矮杆大豆品种Charleston为母本，东北农业大学高蛋白大豆品系东农594为父本及其F2:10代重组自交系的154个株系为试验材料，利用164个在亲本之间表现多态的SSR引物对群体进行了分析，构建了一张大豆遗传图谱。该大豆遗传图谱总长度1 913.5 cM，标记间平均距离为11.89 cM。每个连锁群长度变动在0.4～309.5 cM之间，连锁群上的标记数在2～28个之间。各连锁群上的SSR标记并不是均匀分布的，其中A1、C2、D1a三个连锁群存在标记密集区。与国内外已构建完成的5张大豆遗传图谱比较表明，该图谱与国外的大豆公共遗传图谱对应性较好。

Construction and Analysis of Soybean Genetic Map Using Recombinant Inbred Line of Charleston x Dongnong 594

F2:10 RIL population with 154 lines, crossed by Charleston as female parent and Dongnong 594 as male parent were used in this experiment. One hundred and sixty-four SSR primers were screened with the two parents and amplified on the 154 lines. A new soybean molecular genetic map, named NEAUSRI-GMS, was constructed by Mapmaker. The total length of the soybean genetic map is 1913.5 cM, and the average distance among markers is 11.89 cM. The length of linkage group varied from 0.4 cM to 309.5 cM, and the markers on the linkage group varied from 2 to 28. The distribution of SSR markers on every linkage group is not even. High density region of markers existed on linkage group A1, C2, and D1a. Compared with 5 soybean genetic maps constructed at home and abroad, NEAUSRI-GMS has high homologous with the public genetic map abroad.