鸡FATP1基因多态性与屠体性状的相关分析

 【目的】研究FATP1基因多态性与优质肉鸡屠体性状的相关性,为进一步加快育种进程提供参考依据。【方法】利用PCR-SSCP技术对381只大恒优质鸡FATP1基因的部分外显子进行群体遗传学检测,计算基因频率和基因型频率,对多态性与屠体性状进行相关分析。【结果】在FATP1 P3和P4两个位点上分别存在3种多态以及9种单倍型组合。P3位点不同基因型对活重、屠体重、半净膛、全净膛、胸肌重和腿肌重有显著影响,P4位点不同基因型对腿肌重和腹脂率有显著影响。9种单倍型组合AAMM、AAMN、AGMM、AGMN、AANN、AGNN、GGMM、GGMN和GGNN的频率分别为0.538、0.052、0.265、0.029、0.045、0.037、0.024、0.005和0.005,不同单倍型与腹脂重、胸肌重和胸肌率存在相关性。【结论】鸡FATP1不同基因型和单倍型组合与部分屠体性状和腹脂率存在相关性。

Correlation Analysis Between FATP1 Gene Polymorphism and Carcass Traits in Chicken

【Objective】 The polymorphisms of FATP1 and their correlation to carcass traits in chicken were investigated for purpose of providing molecular maker information to facilitate the breeding efficiency. 【Method】 PCR-SSCP was applied to analyze the polymorphisms of partial exon loci in FATP1 gene in 381 Chinese chicken from Dahen breeding company. Genotype frequencies, allelic frequencies, correlation analysis between the polymorphisms and carcass traits were estimated. 【Result】 The result showed that 3 genotypes and 9 haplotypes combinations were found at the two loci. The genotypes of P3 loci had a significant effect on live weight, carcass weight, semi-eviscerated weight, eviscerated weight, breast weight and leg muscle weight. Different genotypes had a remarkable influence on leg muscle weight and abdominal fat percent in the P4 location. In addition, the frequencies of nine haplotypes combinations were 0.538, 0.052, 0.265, 0.029, 0.045, 0.037, 0.024, 0.005 and 0.005, and different haplotypes combinations affect the breast muscle weight, breast muscle percent and abdominal fat percent. 【Conclusion】 The correlation was detected between different genotypes and haplotypes combinations and partial carcass traits and abdominal fat percent at these two loci.