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外源NO对渗透胁迫下黄瓜种子萌发、幼苗生长和生理特性的影响
引用本文:汤绍虎,周启贵,孙敏,毛薇.外源NO对渗透胁迫下黄瓜种子萌发、幼苗生长和生理特性的影响[J].中国农业科学,2007,40(2):419-425.
作者姓名:汤绍虎  周启贵  孙敏  毛薇
作者单位:西南大学生命科学学院
基金项目:重庆市自然科学基金;西南大学校科研和教改项目
摘    要:【目的】探究外源NO对渗透胁迫下黄瓜(Cucumis sativus L.)种子萌发、幼苗生长和生理特性的作用。【方法】在25% PEG-6000胁迫下,研究0.1和0.5 mmol?L-1SNP(硝普钠)对黄瓜种子萌发、幼苗生长、叶片氧化损伤和保护酶活性的影响。【结果】0.1 mmol?L-1 SNP处理能显著缓解25%PEG-6000的胁迫伤害,使黄瓜种子萌发率提高0.24倍,发芽指数提高0.86倍,活力指数提高3.01倍;幼苗株高、根长和干重分别提高1.15,2.17和1.16倍;叶片Pro含量提高50.99%,SOD,CAT,POD和APX活性分别提高42.39%,66.58%,79.03%和116.39%,MDA含量降低80.50%。与0.1 mmol?L-1 SNP处理相比,0.5 mmol?L-1 SNP处理促进黄瓜种子萌发和幼苗生长,缓解叶片氧化损伤和提高保护酶活性的作用明显或显著降低。【结论】0.1 mmol?L-1 SNP能显著促进渗透胁迫下黄瓜种子萌发和幼苗生长,明显缓解叶片氧化损伤,显著提高SOD等保护酶活性;0.5 mmol?L-1 SNP的有效作用减弱,甚至抑制SOD活性。

关 键 词:黄瓜  种子萌发  一氧化氮(NO)  渗透胁迫  氧化损伤
收稿时间:2006-7-27
修稿时间:2006-07-20

Effects of Exogenous Nitric Oxide on Seed Germination, Seedling Growth and Physiological Characteristics of Cucumber Under Osmotic Pressure
TANG Shao-hu,ZHOU Qi-gui,SUN Min,MAO Wei.Effects of Exogenous Nitric Oxide on Seed Germination, Seedling Growth and Physiological Characteristics of Cucumber Under Osmotic Pressure[J].Scientia Agricultura Sinica,2007,40(2):419-425.
Authors:TANG Shao-hu  ZHOU Qi-gui  SUN Min  MAO Wei
Institution:College of Life Science, Southwest University, Chongqing 400715
Abstract:【Objective】The purpose of this paper is to clarify the protective effects of nitric oxide (NO) on seed germination, seedling growth and leaf oxidative damage in osmotic stress cucumber (Cucumis sativus L.).【Method】Under 25% (W?V-1) PEG-6000 stress condition, the effects of sodium nitroprusside (SNP, an exogenous nitric oxide donor) at the concentration of 0.1 and 0.5 mmol?L-1 on the germination, growth, leaf oxidative damage and protective enzymatic activities in cucumber seedlings were investigated.【Result】The significant effect on the alleviation of osmotic stress damage was observed in the treatment of 0.1 mmol?L-1 SNP. At this concentration, the seed germination rate, germination index and vigor index were increased by 18.89%, 86.03% and 3.02 times respectively, plant height, root length and fresh weight were increased by 1.15, 2.17 and 1.16 times respectively, proline content and activities of SOD, POD, CAT and APX in leaves were increased by 50.99%, 42.39%, 66.58%, 79.03% and 116.39% respectively, while malondialdehyde (MDA) content in leaves was decreased by 4.13 times.【Conclusion】The above results indicated that and dosage effect of exogenous nitric oxide donor (SNP) existed on the promotion of germination and alleviation of osmotic stress in cucumber seedlings, and better alleviation effect on osmotic stress damage was 0.1 mmol?L-1 SNP, which elevated the drought resistance of plants.
Keywords:Cucumber  Seed germination  Nitric oxide(NO)  Osmotic stress  Oxidative damage
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