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白菜雄性不育相关新基因BcMF1的分离及特征分析
引用本文:王华新,王永勤,曹家树,向珣,余小林,叶纨芝.白菜雄性不育相关新基因BcMF1的分离及特征分析[J].中国农业科学,2008,41(4):1119-1127.
作者姓名:王华新  王永勤  曹家树  向珣  余小林  叶纨芝
作者单位:1. 浙江大学蔬菜研究所细胞与分子生物学实验室/农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029:
2. 北京农业生物技术研究中心,北京,100089
基金项目:国家自然科学基金 , 浙江省科研项目 , 浙江省宁波市科技计划
摘    要: 【目的】筛选与白菜雄性不育性状相关的新基因,为研究植物雄性不育的分子机制提供依据。【方法】利用cDNA-AFLP技术分析白菜核雄性不育两用系‘ZUBajh97-01AB ’的表达差异,在可育株群中扩增出1条特异条带BcMF-A15T17,通过RACE和PCR技术扩增得到该基因的cDNA和DNA全长序列,并用Northern杂交验证了该基因的表达特征。【结果】该基因被命名为BcMF1,它的cDNA全长为1 684 bp,基因全长为1 985 bp,BcMF1基因仅在两用系可育株群的中、大花蕾中特异表达。推测的BcMF1蛋白含有471个氨基酸,与拟南芥未知功能蛋白家族DUF1216成员的相似性较高,预测该蛋白是一个定位在胞外的分泌蛋白。【结论】BcMF1基因是一个与白菜细胞核雄性不育相关的新基因。

关 键 词:白菜(Brassica  campestris  L.  ssp.  chinensis  Makino)  雄性不育  基因克隆  BcMF1基因  特征分析
收稿时间:2007-1-10
修稿时间:2007年1月11日

Cloning and Characterization of a New Male Sterility-Related Gene BcMF1 in Brassica campestris L. ssp. chinensis Makino
WANG Hua-xin,WANG Yong-qin,CAO Jia-shu,XIANG Xun,Yu Xiao-lin,YE Wan-zhi.Cloning and Characterization of a New Male Sterility-Related Gene BcMF1 in Brassica campestris L. ssp. chinensis Makino[J].Scientia Agricultura Sinica,2008,41(4):1119-1127.
Authors:WANG Hua-xin  WANG Yong-qin  CAO Jia-shu  XIANG Xun  Yu Xiao-lin  YE Wan-zhi
Abstract:Abstract: 【Objective】This paper aimed to screening a novel gene related with the male sterility in Chinese cabbage for studing the molecular mechanisim of plant male sterility. 【Method】The gene differential expression analysis was performed by cDNA-AFLP in the genic male sterile-fertile line ZUBajh97-01AB of Chinese cabbage, and a differentially expressed cDNA fragment, BcMF-A15T17, was found in fertile line. The full cDNA and DNA sequences of the gene related with BcMF-A15T17 were amplified by RACE and PCR, and the expression characteristic of the gene was proved by Northern blot analysis. 【Result】 The gene, designated as BcMF1, was 1684 bp long in cDNA and1985 bp long in DNA, and specially expressed in middle and big floral buds of the fertile line. The hypothetical protein BcMF1 including 471 amino acids , which was predicted being a extracellular protein in Secretory pathway, had higher similarity in the amino acids sequence to some members from a unknown protein family DUF1216 in Arabidopsis thaliana.【Conclusion】 All the results in this study suggest that BcMF1 is a new gene related with genic male sterility in Chinese cabbage.
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