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转几丁质酶和 β-1,3-葡聚糖酶基因提高棉花对枯萎病和黄萎病的抗性
引用本文:程红梅,简桂良,倪万潮,杨红华,王志兴,孙文姬,张保龙,王晓峰,马存,贾士荣.转几丁质酶和 β-1,3-葡聚糖酶基因提高棉花对枯萎病和黄萎病的抗性[J].中国农业科学,2005,38(6):1160-1166.
作者姓名:程红梅  简桂良  倪万潮  杨红华  王志兴  孙文姬  张保龙  王晓峰  马存  贾士荣
作者单位:[1]中国农业科学院生物技术研究所,北京100081 [2]中国农业科学院植物保护研究所,北京100094 [3]江苏省农业科学院遗传生理研究所,南京210014
摘    要: 枯、黄萎病是世界棉花生产中的两大重要病害。传统育种缺乏抗源,几丁质酶和 -1,3-葡聚糖酶是植物防御体系中的两种防卫因子,两者之间存在协同增效作用。据此构建了4个单价和2个双价基因(分别定位于细胞内或细胞外)的植物表达载体,通过花粉管通道法转化棉花,经PCR和Southern杂交检测以及1996 2000年温室及病圃多代筛选鉴定,已培育出对枯、黄萎病抗性提高的转基因棉花株系。将抗病基因导入国产抗虫棉品种GK19中,还获得了兼抗病、虫的转基因优系。

关 键 词:几丁质酶基因  3-葡聚糖酶基因  转基因棉花  枯、黄萎病抗性
收稿时间:2005-1-17

Increase of Fusarium- and Verticillium-Resistance by Transferring Chitinase and Glucanase Gene into Cotton
Abstract:Fusarium and Verticillium wilt are two important diseases in cotton in the world. Traditional breeding method does not have resistance germplasm. Chitinase and -1,3-glucanase are two factors with synogestic effects involved in the plant defense system. Based on this fact, a series of plant expression vectors either harboring chitinase gene or -1,3-glucanase gene or both were constructed. The two genes are expected to be expressed and localized in vacuole or in extra-cellular space. The transformation of cotton was conducted by pollen-tube pathway method. Molecular analysis of transgenic cotton plants by polymerase chain reaction and Southern hybridization, and successive repeated selection of transgenic cotton lines in greenhouse and fungi-infected nurseries during the period of 1996-2000 demonstrated that the transgenic cotton lines with Fusarium wilt and Verticillium wilt resistance were obtained. Meanwhile, the above anti-fungal genes have been integrated into insect-resistant cotton variety GK19 which resulted in the development of transgenic lines resistant to both cotton boll worm as well as Fusarium and Verticillium wilt.
Keywords:Chitinase gene  3-glucanase gene  Transgenic cotton  Fusarium and Verticillium wilt resistance
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