水稻抗褐飞虱基因Bph14和Bph15的分子标记辅助选择

【研究目的】褐飞虱是危害水稻的三大病虫害之一，利用品种自身的抗性是目前防治褐飞虱最经济有效的方法之一。为了选育抗虫品种，【方法】本研究以携带有抗褐飞虱主效基因Bph14和Bph15的抗虫品系B5为抗源，以优良杂交稻亲本9311和1826为受体材料，通过复交和回交，分别利用与Bph14和Bph15紧密连锁的SSR标记MRG2329和MS5在分离群体中进行分子标记辅助选择，【结果】最终获得一系列目标基因纯合且农艺性状优良的稳定株系。采用苗期群体鉴定技术，对其中的38份重要材料进行了抗虫鉴定，在26份Bph14单基因纯合株系中，92.31%的材料抗性水平在中抗以上，6份Bph15单基因纯合株系全为抗或高抗，聚合有Bph14和Bph15双基因的6份株系抗性都达到了高抗水平。【结论】说明在育种进程中利用与Bph14和Bph15紧密连锁的分子标记开展抗褐飞虱分子标记辅助育种是一种有效的途径。

Marker-Assisted Selection for Brown Planthopper (Nilaparvata lugens St(a)l) Resistance Genes Bph14 and Bph15 in Rice

【OBJECTIVE】Brown Planthopper (BPH), Nilaparvata lugens Stål, is a serious sucking insect pest of rice. Resistance to BPH is an important breeding objective in rice improvement programs.【METHOD】Bph14 and Bph15 have been identified in an introgression line B5 and mapped on chromosome 3 and chromosome 4, respectively. SSR markers MRG2329 linked to Bph14, and MS5 to Bph15, were used to select for some homozygous lines in a segregating population that derived from a cross between B5 and elite restores, 9311 and 1826. The resistance level to BPH of 38 lines was determined by using the mass seedling screening test.【RESULTS】The results indicated that 92.31% of 26 Bph14-single introgression lines showed moderate resistance to high resistance, 6 Bph15-single introgression lines showed resistance or high resistance and 6 pyramid lines (Bph14/Bph15) showed high resistance to BPH insects.【CONCLUSION】The survey revealed that marker-assisted selection is an efficient way for Bph14 and Bph15 selection in rice breeding programs.