日本结缕草成熟种子愈伤组织诱导及植株再生的影响因子分析

 【目的】优化日本结缕草的组织培养体系，提高其植株再生率，建立高频植株再生体系。【方法】以日本结缕草的成熟种子为外植体，分析激素、基本培养基、培养条件、脯氨酸、Cu2+等因子对愈伤组织诱导及植株再生的影响。【结果】（1）培养基中添加脯氨酸能提高愈伤组织诱导率，而BA降低愈伤组织的诱导率，低浓度的NAA(0.1～0.2 mg·L-1)能促进愈伤组织的形成，愈伤组织诱导的最佳培养基为ND＋2,4-D 3.0 mg·L-1＋NAA 0.2 mg·L-1+L-Pro 500 mg·L-1，愈伤组织诱导率达57.3%；（2）愈伤组织继代的最佳培养基为MSD+ 2,4-D 1.0 mg·L-1；（3）愈伤组织分化的最佳培养基为MSD＋NAA 0.3 mg·L-1+KT 0.5 mg·L-1+BA 0.05 mg·L-1 + L-Pro 500 mg·L-1，分化率达36.7%。【结论】优化了日本结缕草的组织培养体系，初步找到了影响其成熟种子愈伤组织诱导及植株再生的主要因子。

Analysis of the Factors Affecting Callus Induction and Plant Regeneration of Zoysia japonica Steud. from Mature Seed

【Objective】In order to optimize tissue culture system of Japanese lawngrass (Zoysia japonica Steud.), and to improve plantlet regeneration rate and to establish high efficient plant regeneration from seed-derived callus, mature caryopses were used as explants. 【Method】The effect of hormone, basical media, cultivation condition, L-proline, Cu2+ on callus induction, maintenance and plantlet regeneration were investigated. 【Result】The results are as follows: (1) The addition of 500 mg·L-1 L-proline to the media was beneficial to callus induction. The addition of BA to the media was disadvantageous to callus induction. Lower concentration NAA increased callus induction rate. The best media for callus induction was ND＋2,4-D 3.0 mg·L-1＋NAA 0.2 mg·L-1 and the ratio of callus induction was 57.3%. (2) The best media for callus subculture was MSD+ 2,4-D 1.0 mg·L-1. (3) The best differentiation media was MSD＋NAA 0.3 mg·L-1+ KT 0.5 mg·L-1+ BA 0.05 mg·L-1 and the ratio of differentiation was 36.7%.【Conclusion】The in vitro system of Japanese lawngrass was optimized and the main factors affecting seed-derived callus induction and regeneration were primarily traced.