| 猪POU1F1第一内含子单核苷酸多态性和生长性状相关性研究 |
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| 引用本文： | 方华,郑友民,李宏滨,杜立新,马月辉.猪POU1F1第一内含子单核苷酸多态性和生长性状相关性研究[J].中国农业科学,2009,42(1):283-289. |
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| 作者姓名： | 方华 郑友民 李宏滨 杜立新 马月辉 |
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| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,北京,100193
2. 中国农业科学院农业质量标准与检测技术研究所,北京,100081 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 【目的】寻找猪POU1F1多态位点和基因表达量之间可能存在的相关性。【方法】以大白猪（英系）和中畜黑猪为试验材料，对POU1F1基因第一内含子多态性进行PCR-SSCP检测分析，同时提取垂体总RNA，对POU1F1、GH、PRL、TSH-?的mRNA进行实时定量荧光PCR检测。【结果】发现一个位于第一内含子1515位点的突变和猪屠宰前日增重相关系数为R=-0.6（P＜0.05），且该位点的替换和屠宰前GH的表达量存在显著相关（R=0.483，P＜0.05）;【结论】T→C的替换将导致GH的mRNA表达量升高，在生长性状上表现为屠宰前日增重降低。
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| 关 键 词： | POU1F1 PCR-SSCP 多态性 SNP位点 |
| 收稿时间： | 2008-1-28 |
Relationship of Single Nucleotide Polymorphism of Intron 1 in Procine POU1 F1 and the Growth Trait |
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| FANG Hua,ZHENG You-min,LI Hong-bin,DU Li-xin,MA Yue-hui.Relationship of Single Nucleotide Polymorphism of Intron 1 in Procine POU1 F1 and the Growth Trait[J].Scientia Agricultura Sinica,2009,42(1):283-289. |
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| Authors: | FANG Hua ZHENG You-min LI Hong-bin DU Li-xin MA Yue-hui |
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| Institution: | Institute of Animal Science, Chinese Academy of Agricultural Sciences |
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| Abstract: | 【Objective】 The relationship between porcine DNA polymorphisms of POU1F1 and mRNA expression was studied. 【Method】 The intron 1, a partial regional polymorphisms of porcine POU1F1 of two breeds of Yorkshare and Zhongxu black, were investigated by PCR-SSCP. Based on the total RNA contracted from animals pituitary, the mRNA quantities of POU1F1, GH, PRL, and TSH-? were masured by RQ-PCR. 【Result】 It was found that a SNP of T→C in intron 1 (site 1515bp), markedly correlated with the daily weight gain (R=-0.6, P＜0.05) and the expression of GH (P＜0.05) before slaughtering. 【Conclusion】The SNP of T→C led to the increase of the GH expression; But the daily weight gain decreasd in the growth trait before slaughtering. |
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| Keywords: | POU1F1 PCR-SSCP |
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