用Envirologix Cry1Ab/Cry1Ac试剂盒快速测定转基因水稻Bt杀虫蛋白含量的研究

 用 Envirologix Cry1Ab/Cry1Ac平板试剂盒检测了两个转 cry1Ab的水稻品系克螟稻 1号 ( KMD1)、克螟稻 2号 ( KMD2 ) ,及未转化的对照品种秀水 11米粒中的 Bt杀虫蛋白含量。结果表明 ,该试剂盒标样制作的标准曲线相关系数为 0 .985～ 0 .998,在 0 .0 5或 0 .0 1水平上显著。同批次试剂盒的不同试验及不同批次试剂盒的测定值均差异不显著。最低检测剂量达 0 .5 ng/g,每次测试时间比 Western dot blotting方法缩短约 2 h。试验表明 ,该试剂盒是定量检测转基因水稻 Bt毒蛋白表达的一种理想产品

Studies on Rapid Quantitative Analysis of Bt Toxin by Using Envirologix Kits in Transgenic Rice

Investigations were done on the usefulness of Envirologix Cry1Ab/Cry1Ac plate kits for quantitative analysis of Bt toxin content in transgenic rice grains Two transgenic rice lines: Kemingdao 1 (KMD1) and Kemingdao 2 (KMD2),transformed with a cry1Ab gene, and their parental variety, C V Xiushui 11, was used as positive and negative samples Results showed that the correlation coefficients as high as 0 985~0 998, significant for 0 05 or 0 01 level, were obtained for linear regression equations by using the appended calibrators of the kits No significant differences were detected for values of same rice sample obtained from different trials or by using different lots of the product (kit) The detectable Bt toxin content by this method could be as low as 0 5 ng/g The Envirologix Kit could be useful for rapid quantitative detection of Bt toxin in rice grains because of its preciseness, simplicity and time saving