AMF对低温胁迫下黄瓜幼苗抗冷性的影响

 【目的】研究丛枝菌根真菌(AMF)对低温胁迫下黄瓜（Cucumis sativus L.）幼苗生长和抗冷性相关生理指标的影响。【方法】以黄瓜品种‘津春2号’为试材，利用人工气候箱进行低温处理（昼/夜，15/10℃）,研究低温胁迫下接种AMF对黄瓜幼苗生长指标、根系活力和叶绿素、丙二醛(MDA)、可溶性蛋白含量及过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响。【结果】低温胁迫下，黄瓜幼苗株高、茎粗、地上部和地下部干鲜重的增长量和根冠比减小，叶绿素含量、根系活力和SOD、CAT活性降低，MDA、可溶性蛋白含量及POD活性升高；在低温胁迫下，接种AMF可以显著提高黄瓜幼苗各生长指标的增长量和根冠比，提高叶片叶绿素、可溶性蛋白含量、根系活力及POD、SOD、CAT活性，降低MDA含量。【结论】低温胁迫下接种AMF可通过促进黄瓜幼苗叶片可溶性蛋白的大量积累和抗氧化酶活性的提高，来降低膜脂过氧化水平，增强黄瓜幼苗对低温胁迫的适应性。

Effects of Arbuscular Mycorrhiz Fungi on Chilling Resistance of Cucumber Seedlings under Low Temperature Stress

【objective】The aim of this experiment was to study the effects of Arbuscular mycorrhiz fungi(AMF) on the changes in growth and physiological in cucumber (Cucumis sativus L.) seedlings.【Method】Cucumber variety ‘Jinchun No.2’ was subjected to low temperature treatment of 15/10℃ at day and Night in the artificial climate chamber. The effects of AMF on the growth and the changes in root activity, chlorophyll, soluble protein and malondialdehyde (MDA) contents, superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) activities under low temperature stress in the seedling leaves were investigated.【Result】Under low temperature stress, the increment of height, stem diameter, fresh weight, dry weight and root-shoot ratio decreased, chlorophyll contents, root activity and SOD ,CAT activities decreased, MDA, soluble protein contents and POD activities increased in cucumber seedlings leaves. AMF-inoculation significantly promoted seedlings growth and chlorophyll, soluble protein contents, POD activities, while reduced MDA contents and SOD,CAT activities.【Conclusion】The results obtained from this experiment showed that under low temperature stress AMF could promote the accumulation of proline, raise the activities of antioxidant enzymes, decrease the membrane lipid peroxidation and improve the adaptability of cucumber seedlings.