紫山药酚酸类化合物鉴定及含量测定

【目的】对产自浙江省台州市黄岩区的紫山药中的酚酸类化合物进行鉴定和含量分析,为其后续天然产物研究提供参考,有利于紫山药及其加工产品的宣传,为这种特色蔬菜更好地走向市场提供帮助。【方法】采用高压液相色谱-二极管阵列技术,对该特种紫山药中的酚酸类化合物进行鉴定,并对其含量进行测定。【结果】酚酸在紫山药中主要以可溶态酚酸形式存在。紫山药中可溶态酚酸类和不可溶态酚酸类均主要为芥子酸和阿魏酸,且芥子酸含量均高于阿魏酸含量。紫山药中可溶态酚酸含量为356.71μg.g-1DW;不溶态酚酸含量为26.92μg.g-1DW。【结论】高压液相色谱-二极管阵列-质谱技术可快速有效分析紫山药中的酚酸类化合物。

Identification and Determination of Phenolic Acids in Purple Yam

【Objective】 As a kind of multipurpose bioactive compound, phenolic acids are widely existed in the plant kingdom. Phenolic acids from purple yam (from Huangyan district, Taizhou city, Zhejiang province) were identified in order to provide references for subsequent analysis on other natural products. 【Method】 Phenolic acids in the purple yam were identified and determined by the method of high performance liquid chromatography (HPLC), photodiode array detection (PAD). 【Result】 The phenolic acids were mainly present in soluble form. Sinapic acid and ferulic acid dominated in both soluble and insolulble fraction and the content of sinapic acid was higher than that of ferulic acid. The total content of soluble phenolic acid was 356.71 μg•g-1 (dry weight) and that of insoluble phenolic acid was 26.92 μg•g-1 (dry weight). 【Conclusion】 HPLC-PAD-MS is a fast and effective method to analyze phenolic acids in purple yam.