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烟草黑胫病菌培养性状的研究
引用本文:马国胜,高智谋.烟草黑胫病菌培养性状的研究[J].中国农业科学,2007,40(3):512-517.
作者姓名:马国胜  高智谋
作者单位:1. 安徽农业大学植物保护学院,合肥,230036;苏州农业职业技术学院园艺与园林系,苏州,215008
2. 安徽农业大学植物保护学院,合肥,230036
基金项目:安徽省高校学科拔尖人才基金;江苏省高校自然科学基金;江苏省教育厅青蓝工程项目
摘    要:【目的】对烟草黑胫病菌的若干培养性状进行研究,进一步了解烟草黑胫病菌的培养特性的稳定性,为烟草黑胫病的相关研究和综合治理提供试验依据。【方法】从安徽10个主要生产烟草的市(县)多点采集发生烟草黑胫病的烟株,采用组织分离法进行病原菌分离,经纯化后获得69个烟草黑胫病菌(Phytophthora nicotianae Breda de Haan var. nicotianae Waterh.)菌株,对其进行游动孢子囊的诱导、配对培养、生长特性测定等进行研究。【结果】烟草黑胫病菌的主要培养性状为:异宗配合,与标准相对交配型菌株配对培养可以产生有性器官;卵孢子球形,满器或不满器,雄器围生,藏卵器小;孢子囊多近球形,乳突明显,有双乳突现象,孢子囊具脱落性,且孢子囊柄短,不对称孢子囊较常见,孢子囊外着生有长短不一的附属丝;菌丝最适生长温度为20~30℃,最高生长温度大于35℃,小于40℃,最低生长温度大于8℃,小于10℃。【结论】供试烟草黑胫病菌的培养性状与前人的研究结论基本一致,初步认为10多年来生态环境和农业措施的变化没有导致烟草黑胫病菌的生长性状发生较大的改变,表明烟草黑胫病菌的培养性状是较为稳定的。

关 键 词:烟草黑胫病  烟草疫霉烟草变种  培养性状  形态特征  异宗配合
收稿时间:2005-11-23
修稿时间:2005-11-23

Cultural Characteristics of Phytophthora Nicotianae var. nicotianae, Pathogen of Tobacco Blank Shank
MA Guo-sheng,GAO Zhi-mou.Cultural Characteristics of Phytophthora Nicotianae var. nicotianae, Pathogen of Tobacco Blank Shank[J].Scientia Agricultura Sinica,2007,40(3):512-517.
Authors:MA Guo-sheng  GAO Zhi-mou
Institution:1.School of Plant Protection, Anhui Agricultural University, Hefei 230036; 2.Department of Gardening and Horticulture, Suzhou Polytechnical Institute of Agriculture, Suzhou 215008
Abstract:Total sixty-nine Phytophthora nicotianae Breda de Haan var. nicotianae Waterhouse isolates were obtained from diseased tobacco stem tissue collected from 10 different localities in Anhui, which Bozhou, Guoyang, Xuxian, Taihe, Lixin, Guzhen, Fengyang, Hefei, Shexian and Wuhu. The main culture character of theses sixty-nine isolates were studied via sporangia abduction, partnership culture in vitro, growth properties mensuration, et al, results showed that these Phytophthora nicotianae Breda de Haan var. nicotianae Waterhouse were heterothallic, with amphigynous antheridia and small oogonia, able to produce oosopora when partnership culture with standard mating type isolates in vitro, and that sporangia were mostly spherical or approximatively spherical, with short caduceus and conspicuously papillate (single or double), easily falling off, and attached some diverse length mycelia-like silk. The asymmetry shape sporangia appeared frequently. The optimum temperature for mycelial growth in vitro was between 20℃ to 30℃, the maximum beyond 35℃ and below 40℃, and the minimum beyond 8℃ and below 10℃.
Keywords:Tobacco blank shank  Phytophthora nicotianae var  nicotianae  Culture characteristics  Morphology  Heterothallic
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