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利用分子标记解析小麦新种质YW243的抗锈病基因
引用本文:刘燕,张增艳,辛志勇,林志珊,杜丽璞,徐惠君,刘丽,陈孝,张改生.利用分子标记解析小麦新种质YW243的抗锈病基因[J].中国农业科学,2006,39(2):295-299.
作者姓名:刘燕  张增艳  辛志勇  林志珊  杜丽璞  徐惠君  刘丽  陈孝  张改生
作者单位:1. 中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,农业部作物遗传育种重点开放实验室,北京,100081;西北农林科技大学农学院,杨凌,712100
2. 中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,农业部作物遗传育种重点开放实验室,北京,100081
3. 西北农林科技大学农学院,杨凌,712100
摘    要: 【目的】对兼抗条锈病、秆锈病、叶锈病、黄矮病、白粉病等5种病害的小麦新种质YW243中抗锈病基因的组成和来源进行解析。【方法】通过系谱推断,利用了抗条锈病基因Yr9、YrX、Yr2、Yr1以及抗秆锈病基因Sr31的特异PCR标记和醇溶蛋白蛋白质电泳图谱,分析YW243及其部分相关亲本材料丰抗8号、丰抗13、陕7859中相关的抗条锈病基因和抗秆锈病基因的有无,解析YW243中抗锈病基因的组成。【结果】YW243含有抗条锈病基因Yr1、Yr2、Yr9、YrX,抗秆锈病基因Sr31、抗叶锈病基因Lr26。Yr1源于亲本陕7859或丰抗13,Yr2源于陕7859或丰抗8号或丰抗13;YrX源于丰抗13;Yr9 、Sr31和Lr26源于陕7859或丰抗8号中。【结论】解析了YW243中抗条锈病基因和抗秆锈病基因的组成,证明YW243是一个含有Yr1、Yr2、Yr9、YrX、Sr31等抗锈病基因的多基因聚合体,这些基因特异的SSR,STS特异标记可用于检测多基因聚合体YW243中目标基因,为在抗锈育种中利用、选择YW243的抗锈基因提供了快捷方法。

关 键 词:小麦  YW243  分子标记  抗锈病基因
收稿时间:08 20 2005 12:00AM
修稿时间:2005-08-202005-12-22

Analysis on the Rust Resistance Genes of a New Wheat Germplasm YW243 by Molecular Markers
LIU Yan,ZHANG Zeng-yan,XIN Zhi-yong,LIN Zhi-shan,DU Li-pu,XU Hui-jun,LIU Li,CHEN Xiao,ZHANG Gai-sheng.Analysis on the Rust Resistance Genes of a New Wheat Germplasm YW243 by Molecular Markers[J].Scientia Agricultura Sinica,2006,39(2):295-299.
Authors:LIU Yan  ZHANG Zeng-yan  XIN Zhi-yong  LIN Zhi-shan  DU Li-pu  XU Hui-jun  LIU Li  CHEN Xiao  ZHANG Gai-sheng
Institution:1.National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding, Crop Institute of Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 2. College of Agronomy, NorthwestAsci-Tech Universitofy of Agricultur and Forestory, Yangling 712100
Abstract:Objective] A new wheat line YW243 is a recently developed new germplasm with multi-resistance to wheat stripe rust, stem rust, leaf rust, wheat yellow dwarf and powdery mildew. In this study, the gene composition of the stripe and stem rust resistance of YW243 was analyzed. In order to clarify the sources of the rust resistance genes of YW243, YW243 and its parents, such as Fengkang 8, Fengkang 13, Shan 7859, Suwon 11 and Heine Hvede, were analyzed by the molecular markers of the resistance genes. Method] The composition and the sources of the stripe and stem rust resistance genes of YW243 were analyzed by using PCR markers of Yr9, YrX, Yr2, Yrl, Sr31 and SDS-PAGE. Result] YW243 possessed at least four stripe rust resistance genes of Yrl, Yr2, Yr9 and YrX, one stem rust resistance gene Sr31. Shan 7859 and Fengkang13 are the sources of Yrl in YW243, Shan7859, Fengkang 8 and Fengkang13 are the sources of Yr2 in YW243, Fengkang 13 is the source of YrX of YW243. Fengkang 8 and Shan 7859 are the sources of Yr9 and Sr31 of YW243.
Keywords:YW243
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