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大麦耐湿性鉴定指标和评价方法研究
引用本文:王军,周美学,许如根,吕超,黄祖六.大麦耐湿性鉴定指标和评价方法研究[J].中国农业科学,2007,40(10):2145-2152.
作者姓名:王军  周美学  许如根  吕超  黄祖六
作者单位:1. 扬州大学江苏省作物遗传生理重点实验室/扬州大学大麦研究所,扬州,225009
2. 澳大利亚塔斯马尼亚大学农学院,塔斯马尼亚,7249
基金项目:国家自然科学基金;大麦遗传资源的中澳合作研究项目
摘    要: 【目的】湿害是大麦生产的主要问题之一,培育耐湿性品种是最经济有效的方法,筛选和鉴定大麦耐湿性资源非常必要。【方法】以大麦(Yerong × Franklin)加倍单倍体(DH)群体165个系为材料,考查湿害处理和对照的株高(PH)、穗长(SL)、穗下节长(TFIL)、单株穗数(SPP)、主穗粒数(GPS)、单株粒重(GWPP)、单株粒数(GPP)、单株干重(DWPP)、千粒重(WTG)、绿叶数(NGL)和叶绿素含量(Chl)。以各性状的耐湿系数作为衡量DH系耐湿性的指标,应用主成分分析法、动态聚类分析和隶属函数法,从样本相关矩阵出发,对大麦DH群体165个系的主要性状进行综合分析。【结果】提出了3个反映大麦主要耐湿性状的主成分及函数式,前两个为穗粒因子,第3个为绿叶数因子;对165个系的耐湿性能力进行基于3个主成分的三维空间下的动态聚类分析和综合评价,将其分成高度耐湿、中度耐湿和极不耐湿3类,数目各为42、93和30。【结论】本研究用新的耐湿性指标和综合评价方法对165个系的耐湿性能力进行了分类、筛选和评价,为进一步的耐湿性QTL研究和大麦耐湿性育种奠定了基础。

关 键 词:大麦  DH群体  耐湿性  主成分分析  动态聚类  综合评价
收稿时间:2006-8-11
修稿时间:2006-08-10

Studies on Selecting Indices and Evaluation Methods for Barley's (Hordeum vulgare L.)Waterlogging Tolerance
WANG Jun,ZHOU Mei-xue,XU Ru-gen,L Chao,HUANG Zu-liu.Studies on Selecting Indices and Evaluation Methods for Barley''''s (Hordeum vulgare L.)Waterlogging Tolerance[J].Scientia Agricultura Sinica,2007,40(10):2145-2152.
Authors:WANG Jun  ZHOU Mei-xue  XU Ru-gen  L Chao  HUANG Zu-liu
Institution:1 Key Laboratory for Crop Genetics and Physiology of Jiangsu Province and the Institute of Barley Research, Yangzhou University, Yangzhou, Jiangsu 225009, China; 2 Tasmanian Institute of Agricultural Research and School of Agricultural Science, University of Tasmania, P.O. Box 46, Kings Meadows, TAS 7249, Australia
Abstract:【Objective】Waterlogging stress is one of the major problems in barley production especially in wetlands. Development of tolerant cultivars is the most cost efficient and environmentally safe way to overcome this problem. The objective of this study was to investigate suitable selection criteria for waterlogging tolerance in barley. 【Method】 165 DH lines derived from a barley cross(Yerong × Franklin) were analysed using principal component analysis, dynamic clustering and subordinate function based on the specimen correlation matrix of 12 traits, including plant height(PH), spike length (SL), the top 1st internode length(TFIL), spike per plant (SPP), grains per spike(GPS), grain weight per plant(GWPP), grains per plant(GPP), straw weight per plant(SWPP), weight of 1000 grains(WTG), number of green leaf (NGL) and Chlorophyll 1-2(Chl 1-2). 【Results】Three principal components and their functional equations, two for spike-grain factor and one for NGL factor, were used to identify lines for waterlogging tolerance, which led to the classification of 165 lines into 3 different levels of tolerance. 30, 93 and 42 lines were classified as waterlogging tolerant, medium tolerant and sensitive, respectively. 【Conclusion】The new selecting indices and comprehensive evaluation method provides a useful tool for further study on QTLs controlling waterlogging tolerance and selecting for waterlogging-tolerance in a breeding program.
Keywords:Barley  Double haploid (DH) population  Waterlogging tolerance  Principal component analysis  Dynamic clustering  Comprehensive evaluation
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