小麦Brock抗白粉病基因近等基因系的培育与分子检测

【目的】通过对2对抗白粉病近等基因系(NILs)Brock/015//京4117、Brock×京4117的遗传背景进行分子检测,使其应用于小麦抗白粉病遗传机理的研究和辅助选择育种。【方法】通过AFLP分子标记方法,检测NILs、Brock、京411遗传背景。同时,对Brock×京4117F2分离群体的抗、感单株进行了分子标记筛选与抗白粉病连锁性分析。【结果】在NILs中发现有"亲二型"、"偏抗病供体亲本Brock型"、"偏轮回亲本京411型"和"交换型"4种AFLP带型。在Brock、NILs和Brock×京411F2分离抗、感单株中,筛选到P15/M14-160AFLP分子标记,这个标记在轮回亲本京411和感病单株中不存在。【结论】AFLP分子检测表明,2对NILs的AFLP带型能反映出Brock和京411双亲遗传背景,这种方法能用于NIL遗传背景检测。P15/M14-160AFLP分子标记与Brock中的抗白粉病基因紧密连锁,为小麦抗白粉病辅助选择育种奠定了基础。

Molecular Evaluation on the Background of Wheat Brock Near Isogenic Lines for Powdery Mildew Resistance

【Objective】 The genetic background of 2 NILs, Brock/015//Jing4117 and Brock×Jing4117, which was to be thought resistant to powdery mildew, were evaluated with molecular detecting and the aim of this work was for breeding a good NILs and for using in wheat disease resistance mechanism studing and marker-assisted seletion breeding. 【Method】 AFLP technique was used in evaluation on genetic background of NILs, Brock and Jing411. Linkage relationships between Brock resistance gene and AFLP characteristic bands were also analyzed in Brock×Jing411 F2 segregation populations. 【Result】 Four types of AFLP band patterns, “parental ditype”, “tendency donor parent Brock type”, “tendency recurrent parent type Jing411”, and “crossingover type”, were identified in NILs. P15/M14-160 was identified in Brock, NILs and Brock×Jing411 F2 segregation resistance individuals. This AFLP band was not found in recurrent parent Jing411 and susceptible individuals. 【Conclusion】 The genetic background of 2 NILs was obtained and good genetic consistency was identified through comparion of NILs and donor parent Brock, recurrent parent Jing411. The results indicated that AFLP molecular detecting method can be used in genetic background evaluation of NIL. AFLP marker P15/m14-160 was highly linked to resistance gene for the powdery mildew in Brock. This study laied a foundation for wheat resistance powdery mildew marker- assited selection breeding.