| 基于PCR技术的玉米丝轴黑粉菌侵染率 |
| |
| 引用本文： | 倪深,肖炎农,王凤格,赵久然,张绍鹏,郑用琏.基于PCR技术的玉米丝轴黑粉菌侵染率[J].中国农业科学,2006,39(9):1804-1809. |
| |
| 作者姓名： | 倪深 肖炎农 王凤格 赵久然 张绍鹏 郑用琏 |
| |
| 作者单位： | 华中农业大学作物遗传改良国家重点实验室 华中农业大学 作物遗传改良国家重点实验室 |
| |
| 基金项目： | 北京市农业育种基础研究理论创新平台项目 |
| |
| 摘 要： | 摘要：以对丝黑穗病高感的黄早四和高抗的Mo17自交系为材料，用丝轴黑粉菌进行接种，采用丝黑穗病病原菌基因组特异引物的PCR技术，分别对幼苗期的根，茎，叶和成熟期的根，茎，叶，雄穗DNA进行PCR扩增，研究病菌对玉米的侵染率及扩展进程。结果表明；特异引物的PCR技术不仅能够对玉米幼苗是否受到病菌的侵染进行早期准确鉴定，而且对丝轴黑粉菌在体内的扩展进程的跟踪研究证明，玉米对丝黑穗病的抗性差异主要表现为抗侵染和抗扩展，抽穗期的雄穗对病樱的形成不能表现出明显的抗性效应。
|
| 关 键 词： | 玉米 丝黑穗 PCR 侵染 扩展 |
| 收稿时间： | 2005-10-24 |
| 修稿时间： | 2005-10-242006-07-05 |
The Research of Sporisorium reiliana's Infection Efficiency and Spreading in Maize Based on PCR Assays |
| |
| NI Shen,XIAO Yan-nong,WANG Feng-ge,ZHAO Jiu-ran,ZHANG Shao-peng,ZHENG Yong-lian.The Research of Sporisorium reiliana''''s Infection Efficiency and Spreading in Maize Based on PCR Assays[J].Scientia Agricultura Sinica,2006,39(9):1804-1809. |
| |
| Authors: | NI Shen XIAO Yan-nong WANG Feng-ge ZHAO Jiu-ran ZHANG Shao-peng ZHENG Yong-lian |
| |
| Institution: | 1. National Key Laboratory of Crop Genetic Improvement/National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070; 2. Maize Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089 |
| |
| Abstract: | Objective] Head smut of maize substantially reduces grain yield. Here we demonstrate a recent survey of the rate of infection of head smut by PCR. Method] Using gene-specific primer derived from the head smut genomics and maize temporal and tissue-specific DNA, the invading process of the pathogen is displayed clearly. Result ] The study shows that the differential resistance of maize against head smut depends mainly on the ability of resistance against infection and spreading. Conclusion ] The above effect disappears in the stamen when the tumor forms during tasseling stage. |
| |
| Keywords: | PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
