新城疫F48E9病毒体外诱导的鸡T淋巴细胞程序性死亡

 应用流式细胞仪及DNA琼脂糖凝胶电泳技术研究了我国鸡新城疫标准强毒F48E9株体外诱导鸡T淋巴细胞的程序性死亡现象。同时,对其灭活毒以及从该毒株提取的囊膜蛋白诱导鸡T淋巴细胞的程序性死亡现象进行了观察,并以健康SPF鸡胚尿囊液作为对照。结果发现,F48E9株及其灭活毒体外均可诱导鸡T淋巴细胞发生程序性死亡,而从病毒中提取的囊膜蛋白及SPF鸡胚尿囊液无此特性。新城疫F48E9株体外诱导鸡T淋巴细胞程序性死亡这一现象初步表明,新城疫强毒F48E9株感染鸡T淋巴细胞数量的减少和功能的降低以及由此所致的感染鸡免疫功能的降低可能和病毒诱导鸡T淋巴细胞发生程序性死亡有关。

The Programmed Cell Death of Chicken T Lymphocytes Induced by Newcastle Disease Virus in vitro

Flow cytometry(FCM) and electrophoretic analysis of DNA fragmentation were used to study the programmed cell death(PCD) of chicken T lymphocytes from SPF chickens which were induced by newcastle disease virus(NDV) F48E9 strain. The inactivated F48E9, the capsule protein from F48E9 were observed and the allantoic fluid from SPF chickens was used as a control. It was demonstrated that NDV F48E9 strain and the inactivated virus could induce obvious PCD but the later two could not. According to the results we could predict that the PCD induced by NDV would be one of the major results of decrease of the numbers and disfunction of T lymphocytes in NDV infected chickens.