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干旱胁迫下红麻叶片的差异蛋白表达分析
引用本文:祁建民,姜海青,陈美霞,徐建堂,马红勃,方平平,林荔辉,陶爱芬,陈伟.干旱胁迫下红麻叶片的差异蛋白表达分析[J].中国农业科学,2012,45(17):3632-3638.
作者姓名:祁建民  姜海青  陈美霞  徐建堂  马红勃  方平平  林荔辉  陶爱芬  陈伟
作者单位:1.福建农林大学作物遗传育种与综合利用教育部重点实验室,福州 350002; 2.福建农林大学农业部东南黄红麻科学观测试验站,福州 350002; 3.江苏省海头高级中学,江苏连云港 222023; 4.宁德师范学院生物工程系,福建宁德 352100; 5.南京农业大学农学院/作物遗传与种质创新国家重点实验室,南京 210095
基金项目:福建省发改委“五新”项目(SCZ10007);福建省科技厅南方黄/红麻种质资源共享平台项目(2010N2002);农业部黄/红麻东南科学观测实验站建设项目(农科教发2011-9)
摘    要:【目的】研究耐旱性高的红麻品种在干旱胁迫条件下的蛋白质表达,揭示红麻耐旱性的生理机制。【方法】以鉴定出的耐旱性红麻品种GA42为材料,在苗期(五叶期)设置正常供水与控水比较试验,运用双向电泳分析红麻在干旱胁迫和正常供水条件下叶片蛋白质组的动态变化。【结果】对2-DE图谱分析后发现,在干旱胁迫下出现65个差异表达蛋白质点,选用表达量明显上调的9个蛋白质点,通过MALDI-TOF-TOF MS分析和数据库检索,鉴定出6个差异表达蛋白的功能,分别是2个核酮糖-1,5-二磷酸羧化酶(Rubisco)或其大亚基(所有植物进行光合碳同化的关键酶)、1个Rubisco活化酶(广泛存在于植物中调节Rubisco活性的酶)、1个二甲基萘醌甲基转移酶(一种参与甲基转移反应的辅酶)、1个推定的胞质型谷氨酰胺合成酶(参与高等植物氨同化过程的关键酶)、1个ATP合酶β亚基(在活性细胞中起着将其它能量合成为生物能量通货-ATP的能量转换作用)。【结论】揭示了红麻GA42表现出较强的耐旱性与上述6个差异表达蛋白质点明显上调有关。

关 键 词:红麻  干旱胁迫  差异蛋白  双向电泳
收稿时间:2012-03-19

Differential Protein Analysis of Kenaf Leaves Under Drought Stress
QI Jian-min,JIANG Hai-qing,CHEN Mei-xia,XU Jian-tang,MA Hong-bo,FANG Ping-ping,LIN Li-hui,TAO Ai-fen,CHEN Wei.Differential Protein Analysis of Kenaf Leaves Under Drought Stress[J].Scientia Agricultura Sinica,2012,45(17):3632-3638.
Authors:QI Jian-min  JIANG Hai-qing  CHEN Mei-xia  XU Jian-tang  MA Hong-bo  FANG Ping-ping  LIN Li-hui  TAO Ai-fen  CHEN Wei
Institution:1(1Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Fujian Agriculture and Forestry University,Fuzhou 350002;2Ministry of Agriculture,Jute/Kenaf Scientific Observation Southeast Experiment Station,Fujian Agriculture and Forestry University,Fuzhou 350002;3Jiangsu Haitou Senior High School,Lianyungang 222023,Jiangsu;4Biological Engineering Department,Ningde Normal University,Ningde 352100,Fujian;5College of Agriculture,Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095)
Abstract:【Objective】 The analysis of stress-responsiveness in kenaf is an important route to the discovery of genes conferring stress tolerance. Proteomic analysis provides a broad view of plant responses to stress at the level of proteins.【Method】A drought-resistant kenaf variety GA42 has been identified as materials by setting the normal water supply and water control comparison test at seeding stage, stress-induced proteome changes were analyzed by two-dimensional gel electrophoresis.【Result】In total, 9 up-regulated protein spots reproducibly presented differential expression patterns on 2-DE maps. Six spots were identified and confirmed by MALDI-TOF-TOF MS, including 2 ribulose-1, 5-bisphosphate carboxylase or large chain (a key enzyme in the first major step of carbon fixation), 1 rubisco activase (a enzyme could regulate the activity of rubisco existing widely in plants), 1 dimethylmenaquinone methyltransferase (a coenzyme participates in methyl transfer reactions), 1 putative plastidic glutamine synthetase (a key enzyme participates in ammonia assimilation in higher plants), and 1 ATP synthase subunit β (an important enzyme that provides energy for the cell to use through the synthesis of ATP, which is the most commonly used “energy currency” of the cells from most organisms). 【Conclusion】 This study preliminarily showed these six protein spots are up-regulated which are associated with GA42 resistance under drought stress.
Keywords:kenaf (Hibiscus cannabinus L    drought stress  differential protein  2-DE
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