RFLP揭示的籼粳基因组多态性

采用28个RFLP籼粳特异性探针和6个SSR籼粳特异性标记对水稻不同品种进行了籼粳基因组多态性分析，结果表明，这些标记分布在水稻12条染色体上，能对水稻品种进行籼粳稻分子分类。由于水稻基因组序列图的完成和释放，使得这些籼粳特异性标记揭示的基因组多态性可以在基因组序列水平上进行分析和验证。本研究以RZ906探针和RM405为例分析了籼粳特异性其及序列分子生物学基础。

Genome Polymorphisms Between Indica and Japonica Revealed by RFLP

Objective] Revealing the genome polymorphisms between Indica and Japonica subspecies. Method] RFLP markers, which are distributed on 12 chromosome of rice, were used to analyze Indica-Japonica differentiation in different rice varieties. The same time, genome sequence variations of screened loci were analyzed by bioinformatics method. Result] Twenty-eight RFLP probes, which can classify Indica-Japonica rice, were confirmed. Subspecies genome polymorphisms of screened loci were found by analyzing the publication of the genome sequences data of rice. Conclusion ] The study indicated that these screened markers can be used for classifying Indica-Japonica subspecies. With the publication of the genome sequences of rice, marker polymorphisms between Indica and Japonica subspecies can be revealed by genome sequence differentiation.