基于cytb基因探讨家绵羊和多角绵羊的系统发育

 【目的】研究中国家绵羊起源进化以及多角绵羊和普通家绵羊的亲缘关系。【方法】测定8个省区42个绵羊个体的细胞色素b基因全序列。【结果】本试验的绵羊个体可定义为40个单倍型,单倍型多样度为0.997±0.006,核苷酸多样度为0.793%。中国绵羊群体可分为4个支系(A、B、C 和D),其中支系D首次在中国绵羊群体中被检测到;支系B与支系C的差异最大,分化时间大约为595±150万年。多角绵羊与家绵羊聚在一起,而与野绵羊亲缘关系较远,分属于支系A、B和D。【结论】系统发育分析表明,中国绵羊有多个母系起源或经过多次驯化,未发现盘羊、东方盘羊和亚洲摩佛伦羊与绵羊母系祖先形成相关的分子证据。

Phylogenetics of Domestic Sheep and Multi-Horned Sheep Based on cytb Gene

【objective】 The aim of the study is to investigate the origin and evolution of Chinese domestic sheep (ovis aries) and the relationship between domestic sheep and multi-horned sheep, 【Method】 The complete cytb sequences of 42 individuals from 8 provinces were sequenced. 【Result】 The result showed that totally 40 haplotypes were detected, and Haplotype diversity (Hd) and nucleotide diversity (?) were 0.997±0.006 and 0.793%, respectively. The neighbour-joining tree indicated that there are four distinct lineages (A, B, C and D) in Chinese domestic sheep. It is firstly reported that lineage D were detected in Chinese sheep. And lineages B and C had the highest variance value, which diverged from each other about 595±150 million years. The multi-horned sheep belonged to lineages A, B and D, which had closer relationship with domestic sheep than wild sheep. 【Conclusion】 Analysis of phylogenesis shows that Chinese sheep have multiple maternal origins or ever experienced several times domestication possibly. There is no evidence that urial, argali and asian mouflon had molecular contribution to the maternal ancestor of Chinese sheep.