长白-蓝塘猪资源群第6号染色体的QTL检测

 以国外猪种长白猪和华南地区优良地方猪种蓝塘猪为亲本 ,建立了用于QTL定位研究的F2 资源群。应用 6号染色体上的 7个微卫星遗传标记和USDA MARC 2 .6公、母猪平均遗传连锁图谱 ,采用区间定位方法进行基因组扫描。结果表明 ,在 38～ 4 1cM间发现影响猪胴体A点膘厚的QTL ,达到染色体极显著水平 (P <0 .0 1) ,该QTL与MN0 0 7标记紧密连锁 ,其加性方差解释F2 表型方差的比例为 5 .90 %。 6 0～ 6 5cM间发现影响猪胴体瘦肉率的QTL ,达到染色体极显著水平

QTL Detection on Chromosome 6 in Landrace-Lantang Pig Resource Population

A resource population constructed by F 2 design with Landrace and Chinese indigenous Lantang pigs was used in this study. Seven microsatellite DNA markers on chromosome 6 and USDA-MARC 2.6 pig genetic linkage map were used for interval QTL mapping. The results revealed that at the position of 38-41 cM there was a chromosome-wide highly significant QTL affecting carcass backfat A thickness (P<0.01),which was closely linked with MN007 and the ratio of QTL additive variance to F 2 phenotypic variance was 5.90%. At the position of 60-65 cM there were two chromosome-wide significant QTLs affecting carcass lean percentage (P<0.01) and skin and fat percentage (P<0.05), which were closely linked with MN003 and the ratio of QTL additive variance to F 2 phenotypic variance were 18.44% and 3.75% respectively. At the same position, there was a single-point QTL also closely linked with MN003 and highly significantly (P<0.01) affecting carcass lean. In addition, there were two chromosome-wide highly significant (P< 0.01) QTLs affecting meat color and marbling, which were closely linked with MN13 at the position of 70-75 cM and the ratio of QTL additive variance to F 2 phenotypic variance were 14.05% and 1.77% respectively.