转染技术在顶复门原虫研究中的应用

 自1993年转染技术首次在弓形虫中成功报道以来,顶复门原虫的转染研究得到了快速发展,不仅在弓形虫、疟原虫和住肉孢子虫等实现了稳定转染,而且在体外细胞上生长效率较低的柔嫩艾美耳球虫也相继实现了瞬时转染和稳定转染。顶复门原虫转染技术的日渐成熟及其在顶复门原虫研究中的应用,为顶复门原虫基因功能和免疫学等领域研究提供了新思路和新的有力工具。本文结合本课题组近几年来的研究成果,综述了转染技术及其在顶复门原虫基因表达调控、基因功能、耐药性分子机理、药物敏感性试验和免疫学等研究中应用的最新进展。

Transfection Technology and Its Application in the Investigation of Apicomplexan Protozoa

Since transient transfection of Toxoplasma gondii was first reported in 1993, stable transfections of apicomplexa such as T. gondii, Plasmodium species and Sarcocystis neurona have been achieved later, moreover, both the in vitro transient and in vivo stable transfection systems of Eimeria tenella, which do not undergo re-cycling of any life cycle stages, has been developed successfully. With the rapid development of transfection technology in Apicomplexan parasites, genetic manipulation provides a powerful tool for the study of the biochemical and cell biology of Apicomplexa. This paper has reviewed the latest progress of transfection technology and its application in gene regulation, the function of gene, the nature of drug resistance, drug sensitivity test and immunology of Apicomplexan parasites in combination with the related researches in authors’ laboratory in past years.