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烟草种质资源遗传多样性与亲缘关系的ISSR聚类分析
引用本文:梁景霞,祁建民,方平平,王涛,陈顺辉,周东新,陶爱芬,梁康迳,吴为人.烟草种质资源遗传多样性与亲缘关系的ISSR聚类分析[J].中国农业科学,2008,41(1):286-294.
作者姓名:梁景霞  祁建民  方平平  王涛  陈顺辉  周东新  陶爱芬  梁康迳  吴为人
作者单位:1. 福建农林大学生命科学学院/作物科学学院,福州,350002
2. 福建南平烟草公司,福建南平,353000
3. 福建省烟草科学研究所,福州,350003
4. 龙岩烟草科学研究所,福建龙岩,364000
基金项目:国家烟草专卖局资助项目
摘    要: 【目的】深化烟草种质资源遗传多样性研究,为种质利用改良烟草提供科学依据。【方法】应用ISSR分子标记方法,以烟草属6个种共96份有代表性的种质为材料,进行遗传多样性与亲缘关系分析。【结果】(1)从90个ISSR引物中筛选出18个多态性引物对全部实验材料进行PCR扩增,共获得314条稳定的条带,其中多态性条带299条(占95.2%)。(2)应用Nei-Li相似系数法估算了96份材料间的遗传相似系数(GS),其GS在0.28~0.97之间,遗传多样性丰富。其中普通烟草栽培品种间的GS在0.62~0.98之间,平均为0.78,品种间的遗传基础相对狭窄,普通烟草栽培种与野生种及黄花烟的GS在0.28~0.58之间,平均为0.42,种间遗传差异较大;(3)对91份普通烟草栽培品种的分子系统聚类分析表明:地理来源相同的部分品种有相对聚合现象或少部分国内外品种和类型出现交叉聚类,与其亲缘关系较近有关;个别品种自行一类,与其特异的遗传基础差异较大有关。【结论】ISSR是一种较有效、稳定和可靠的分子标记,本研究可为烟草育种的亲本利用及开展烟草遗传连锁图的构建和核心种质指纹图谱的绘制提供重要的科学依据。

关 键 词:烟草  种质资源  ISSR  遗传多样性  亲缘关系
收稿时间:2006-05-22
修稿时间:2006-11-13

Genetic Diversity and Genetic Relatives Analysis of Tobacco Germplasm Based on Inter-Simple Sequence Repeat(ISSR)
LIANG Jing-xia,QI Jian-min,FANG Ping-ping,WANG Tao,CHEN Shun-hui,ZHOU Dong-xin,TAO Ai-fen,LIANG Kang-jing,WU Wei-ren.Genetic Diversity and Genetic Relatives Analysis of Tobacco Germplasm Based on Inter-Simple Sequence Repeat(ISSR)[J].Scientia Agricultura Sinica,2008,41(1):286-294.
Authors:LIANG Jing-xia  QI Jian-min  FANG Ping-ping  WANG Tao  CHEN Shun-hui  ZHOU Dong-xin  TAO Ai-fen  LIANG Kang-jing  WU Wei-ren
Abstract:【Objective】The studies of genetic diversity of Nicotiana tabacum accessions and relationships among them are essential for the utilization of them.【Method】Inter-simple sequence repeat (ISSR) amplification analysis was used to determine the degree of intra- and inter-specific genetic variation in the genus Nicotiana,91 cultivars (Nicotiana tabacum L.) and five wild Nicotiana species were analyzed. 【Result】1)using eighteen primers screened from 90 ISSR primers,a total of 314 DNA fragments were amplified,of which 299 (PPB= 95.20%) were polymorphic,It showed that ISSR technique was capable of detecting more polymorphic than other makers; 2)The genetic similarity among 96 accessions ranged from 0.28 to 0.97.The genetic similarity coefficients between 5 wild species and cultivar was 0.28~0.58, average value was 0.42, while among cultivars was 0.62~0.98, average value was 0.78. The analysis indicated that a greater amount of genetic polymorphism exists among species of Nicotiana than among cultivars,and the genetic basis was comparatively narrow among the cultivars, except only a few of different varieties; 3) Cluster analysis by unweighted pair group method of arithmetic means(UPGMA)suggested that the 5 wild species were genetically different from the other 91 cultivars at the level of D=0.48,exhibited high levels of interspecific genetic variation.4) The cluster analysis only to 91 accessions of cultivar indicated that three small clusters and one big cluster was found at the level of D=0.40.The big cluster was consist of many different groups. Some groups were consist of cultivars that were primarily based upon the same geographic origin or manufactuing quality trait,maybe all these cultivars has similar genotype;Morever,some groups were consist of cultivars that had different geographic origin or manufactuing quality trait,that because there was close relationship among these cultivars;A few other groups were consist of only one cultivar,it showed that these cultivars has a genetic base different from other cultivars.In a word,the marker of ISSR was useful in revealing the genetic diversity and relationship among tobacco germplasm resourse.【Conclusion】This research providing a scientific basis for broadening the gene resources of cultivar through germplasm innovation and tobacco breeding,as well as construction of DNA fingerprint of core germplasm and molecular genetic linkage map of tabacco.
Keywords:Tobacco  Germplasm resource  ISSR  Genetic diversity  Genetic relationship
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