| 大白菜分子遗传图谱的构建与分析 |
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| 引用本文： | 于拴仓,王永健,郑晓鹰. 大白菜分子遗传图谱的构建与分析[J]. 中国农业科学, 2003, 36(2): 190-195 |
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| 作者姓名： | 于拴仓 王永健 郑晓鹰 |
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| 作者单位： | 北京市农林科学院蔬菜研究中心,北京,100089 |
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| 基金项目： | 北京市蔬菜种质改良重点实验室资助项目 |
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| 摘 要： | 利用不同生态型的大白菜 (BrassicacampestrisL .ssp .pekinensis)栽培种高代自交系 177和 2 76杂交获得的 10 2份F6重组自交系 ,通过对AFLP和RAPD两种分子标记进行遗传分析 ,构建了包含 17个连锁群 ,由 35 2个遗传标记组成的大白菜连锁图谱 ,其中包括 2 6 5个AFLP标记和 87个RAPD标记。该图谱覆盖基因长度2 6 6 5 .7cM ,平均图距 7.6cM。AFLP标记对于增加图谱密度效率较高 ,但容易出现聚集现象 ,从而造成连锁群上有较大的空隙。连锁群上有 13.92 %的分子标记表现偏分离 ,偏分离标记在连锁群上聚集出现。
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| 关 键 词： | 大白菜 遗传图谱 AFLP RAPD |
| 修稿时间： | 2002-09-10 |
Construction and Analysis of a Molecular Genetic Map of Chinese Cabbage (Brassica campestris L. Ssp. Pekinensis) |
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| YU Shuan cang,WANG Yong jian,ZHENG Xiao ying. Construction and Analysis of a Molecular Genetic Map of Chinese Cabbage (Brassica campestris L. Ssp. Pekinensis)[J]. Scientia Agricultura Sinica, 2003, 36(2): 190-195 |
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| Authors: | YU Shuan cang WANG Yong jian ZHENG Xiao ying |
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| Abstract: | A molecular genetic map of Chinese cabbage was constructed with a 102 F 6 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276 by using AFLP and RAPD markers. The map consists of 17 linkage groups, which include 352 genetic markers, and covers 2 665.7 cM with an average genetic distance of 7.6 cM. AFLP marker is efficient for map construction while it easily form clusters to cause big gaps in map. A total of 13.92% distorted markers distributed in the map. The molecular genetic map is fundamental to gene localization, comparative genomics and QTL mapping of important agrono mic traits. |
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| Keywords: | Chinese cabbage Molecular genetic map AFLP RAPD |
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