黄淮地区大豆花叶病毒株系的鉴定与分布

2001-2002年采集了黄淮地区四省28个县市的大豆病样591份，经初步繁殖鉴定、生物纯化及组织印迹检测，得到了50个SMV毒株, 采用王修强等筛选的10个鉴别寄主进行接种鉴定，检测到SC-3～SC-9等7个株系群，发现1个新的株系群SC-10。综合本单位1998－2002年的结果，黄淮地区(河南、山东、安徽北、江苏北) 在SC-1~SC-10中，除SC-2未发现外，9个株系群中，以SC-3和SC-7为主，分别占29.21% 和23.60%，SC-4和SC-8其次（10.11%、8.99%）。在各省分布上，河南省有7个株系群，SC-3、SC-4为主，SC-5、SC-7其次；山东省4个株系群，以SC-3为主，SC-8也占相当比重；皖北7个株系群，以SC-7和SC-9为主，其次SC-10；苏北8个株系群，以SC-3和SC-7为主，其次SC-8。按株系群看，SC-3、SC-4各省均有；SC-5、SC-6 、SC-7 在河南、皖北、苏北3地；SC-1、SC-8在河南、山东、苏北3地；SC-9只在皖北发现；SC-10在皖北、苏北2地发现；SC-2在黄淮未发现。

Identification and Distribution of SMV Strains in Huang-Huai Valleys

In 2001-2002, 591 diseased specimens of soybean leaves collected from Huang-Huai Valleys were purified, then from them fifty SMV isolates were acquired by using direct tissue blotting immunoassay (DTBIA). Based on their response to ten differential hosts screened by Wang et al., seven SMV strain groups, SC-3 ~ SC-9, were identified and a new strain group SC-10 was discovered. The analysis of the data in present study (2001-2002) combined with those from Wang and Yang (1998-2000) showed that in Huang-Huai Valleys (Henan, Shandong, north Anhui and north Jiangsu) nine strain groups among SC-1~SC-10, except SC-2, existed with the major ones being SC-3 and SC-7 accounting for 29.21% and 23.60%, respectively, and second ones being SC-4 and SC-8 accounting for 10.11% and 8.99%, respectively. In Henan there were seven strain groups with SC-3、SC-4 as major ones and SC-5、SC-7as second ones; in Shandong, four ones with SC-3 as major one and SC-8 as second one; in north Anhui, seven ones with SC-7 and SC-9 as major ones and SC-10 as second one; in north Jiangsu, eight ones with SC-3 and SC-7 as major ones and SC-8 as second one. As for the individual strain groups, SC-3 and SC-4 existed in all the four provinces, SC-5, SC-6 and SC-7 in Henan, north Anhui and north Jiangsu, SC-1 and SC-8 in Henan, Shandong and north Jiangsu, SC-9 only in north Anhui, SC-10 only in north Anhui and north Jiangsu, while SC-2 was not found in Huang-Huai Valleys.