6种酚酸类物质对平邑甜茶幼苗根系线粒体及抗氧化酶活性的影响

 【目的】研究对羟基苯甲酸、间苯三酚、丁香酸、苯甲酸、咖啡酸和阿魏酸等6种酚酸类物质对平邑甜茶幼苗根系线粒体和抗氧化酶活性的影响。【方法】育苗基质栽培平邑甜茶幼苗，分别用50 mL浓度为0.8、4.0、20.0 mmol•L-1的6种酚酸类物质处理，测定平邑甜茶幼苗鲜重、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的活性、线粒体膜通透性转运孔（MPTP）开放程度、膜流动性、细胞色素Cyt c/a比值的变化。【结果】0.8、4.0、20.0 mmol•L-1的6种酚酸类物质均抑制了平邑甜茶植株生长，降低了SOD、POD、CAT活性；使线粒体MPTP开放程度增大，线粒体膜流动性降低，细胞色素Cyt c/a比值下降，使线粒体受到不同程度的损伤。与其它处理比，20.0 mmol•L-1苯甲酸处理的抑制效果最显著。【结论】0.8、4.0、20.0 mmol•L-1的6种酚酸类物质都抑制了平邑甜茶抗氧化酶活性和线粒体功能，且浓度越高抑制作用越强；苯甲酸比另外5种酚酸类物质具有更强的抑制作用。

Effects of Phenolic Acids on Mitochondria and the Activity of Antioxidant Enzymes in Roots of Seedlings of Malus hupehensis Rehd.

【Objective】Experiments were conducted to study the effects of ρ-hydroxybenzoic acid, phloroglucinol, syringic acid, benzoic acid, caffeic acid and ferulic acid on the functions of mitochondria and the activities of antioxidant enzymes in roots of Malus hupehensis seedlings which were apples common stocks.【Method】 Malus hupehensis seedlings were planted in bowl and were teated with 50 mL of 0.8, 4.0, 20.0 mmol•L-1 of the six kinds of phenolic acids at 3-day intervals. Fresh weight, activities of superoxidase (SOD), catalase (CAT) and peroxidase (POD), the opening of mitochondrial permeability transition pores (MPTP), membrane fluidity and cytochrome c/a were determined. 【Result】 Treatments with 0.8, 4.0, 20.0 mmol•L-1 of six kinds of phenolic acids all inhibited the growth of Malus hupehensis seedlings, reduced the activities of SOD, POD, and CAT, increased the opening of MPTP, decreased membrane fluidity and Cyt c/a, and impaired mitochondria in a sense. Benzoic acid at 20.0 mmol•L-1 had more significant inhibitions than other treatments. 【Conclusion】 The six kinds of phenolic acids at 0.8, 4.0, 20.0 mmol•L-1 could inhibit the activities of antioxidant enzymes and mitochondrial functions, and the inhibition depended on dose of phenolic acids. At the same concentrations, benzoic acid has stronger inhibition than other five phenolic acids.