首页 | 本学科首页   官方微博 | 高级检索  
     检索      

玉米幼胚和源于幼苗的幼嫩叶段愈伤诱导及其植株再生的比较
引用本文:陈靖,董浩,马海珍,孙全喜,刘江,亓宝秀,李新征,董树亭.玉米幼胚和源于幼苗的幼嫩叶段愈伤诱导及其植株再生的比较[J].中国农业科学,2011,44(17):3676-3682.
作者姓名:陈靖  董浩  马海珍  孙全喜  刘江  亓宝秀  李新征  董树亭
作者单位:山东农业大学作物生物学国家重点实验室
基金项目:国家重点基础研究发展计划(2011CB100100); 国家自然科学基金(30871476,30970222); 山东省良种工程产业化项目(鲁农粮种字[2007]7号)
摘    要: 【目的】以育种上常用自交系的幼胚和源于幼苗的幼嫩叶段为外植体,研究它们在愈伤诱导及植株再生之间的区别与联系,为建立以叶段为外植体的另一高效稳定的玉米组培体系提供技术参考。【方法】选用6个育种上常用自交系,以幼胚和幼嫩叶段为外植体分别进行愈伤诱导及植株再生,研究2,4-D对不同外植体初级愈伤组织诱导的影响,对不同来源的6个自交系分别统计诱导率及分化率,并观察愈伤组织的形态。【结果】不同外植体对2,4-D浓度的要求不同,且幼嫩叶段相对幼胚较难诱导愈伤且需要的2,4-D浓度更高。同一基因型的幼胚和幼嫩叶段形成的愈伤形态基本无区别,不同基因型之间愈伤形态有差异。不同来源的6个基因型的愈伤诱导率、分化率也存在差异。【结论】通过再生过程中幼胚和叶段多方面的比较,筛选到3个诱导率较高且愈伤形态优良的基因型齐319、Mo17和鲁原92。

关 键 词:玉米  (Zea  mays  L.)    幼胚    幼嫩叶段    愈伤组织诱导    分化
收稿时间:2011-03-04

Comparison of Induction and Regeneration of Embryogenic Callus Initiated from Immature Embryos and Seedling-Derived Young Leaf Segments of Maize
CHEN Jing,DONG Hao,MA Hai-zhen,SUN Quan-xi,LIU Jiang,QI Bao-xiu,LI Xin-zheng,DONG Shu-ting.Comparison of Induction and Regeneration of Embryogenic Callus Initiated from Immature Embryos and Seedling-Derived Young Leaf Segments of Maize[J].Scientia Agricultura Sinica,2011,44(17):3676-3682.
Authors:CHEN Jing  DONG Hao  MA Hai-zhen  SUN Quan-xi  LIU Jiang  QI Bao-xiu  LI Xin-zheng  DONG Shu-ting
Institution:CHEN Jing,DONG Hao,MA Hai-zhen,SUN Quan-xi,LIU Jiang,QI Bao-xiu,LI Xin-zheng,DONG Shu-ting(State Key Laboratory of Crop Biology,Shandong Agricultural University,Taian 271000,Shandong)
Abstract:【Objective】 Using immature embryos and seedling-derived leaf segments of maize inbred lines that are commonly used in breeding as explants, the difference and correlation between these two explants in terms of their callus induction and regeneration were studied. The aim of this study is to provide a protocol for the establishment of an efficient and stable regeneration system in maize using leaf segments as an alternative explant. 【Method】 Six inbred lines of maize (Zea mays L.) that routinely used in breeding were tested in this study. The initiation, maintenance and differentiation of callus from their immature embryos and seedling-derived leaf segments were compared. The effect of 2,4-D on primary callus induction of different explants from all these six lines was studied. The callus induction and differentiation frequency were recorded, and the callus morphology was also observed. 【Result】 Different explants required different concentrations of 2,4-D to induce callus formation. Generally, the leaf segment was more difficult to induce and also required higher concentration of 2,4-D compared with immature embryos. The callus induction and differentiation rate of these two explants of all six genotypes were very different although that of the same genotype were similar. 【Conclusion】 Three genotypes, Qi 319, Mo17 and Luyuan 92, were selected after screening through six lines for their performance in higher callus induction rate and excellent morphology.
Keywords:maize(Zea mays L  )  immature embryos  young leaf segments  embryogenesis callus induction  differentiation  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国农业科学》浏览原始摘要信息
点击此处可从《中国农业科学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号